Regulation of TiO2 nanotubes on titanium implants to orchestrate osteo/ angio-genesis and osteo-immunomodulation for boosted osseointegration

作者全名："Tao, Bailong; Lan, Haiyang; Zhou, Xiaolin; Lin, Chuanchuan; Qin, Xian; Wu, Meng; Zhang, Ye; Chen, Sinan; Guo, Ai; Li, Kai; Chen, Lixue; Jiao, Yang; Yi, Weiwei"

作者地址："[Tao, Bailong; Zhang, Ye; Chen, Sinan; Guo, Ai; Li, Kai; Chen, Lixue] Chongqing Med Univ, Affiliated Hosp 1, Lab Res Ctr, Chongqing 400016, Peoples R China; [Lan, Haiyang] Army Med Univ, Daping Hosp, Dept Spine Surg, Chongqing 400000, Peoples R China; [Zhou, Xiaolin] Army Med Univ, Daping Hosp, Dept Gen Practice, Chongqing 400000, Peoples R China; [Lin, Chuanchuan] Army Med Univ, Affiliated Hosp 2, Dept Blood Transfus, Lab Radiat Biol, Chongqing 400037, Peoples R China; [Qin, Xian] Chongqing Med Univ, Women & Childrens Hosp, Chongqing 401147, Peoples R China; [Wu, Meng] Changzhou Univ, Sch Mech Engn & Rail Transit, Changzhou 213164, Peoples R China; [Jiao, Yang] Seventh Med Ctr PLA Gen Hosp, Dept Stomatol, Beijing 100700, Peoples R China; [Yi, Weiwei] Chongqing Med Univ, Affiliated Hosp 1, Dept Rehabil Med, Chongqing 400016, Peoples R China"

通信作者："Tao, BL (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Lab Res Ctr, Chongqing 400016, Peoples R China.; Jiao, Y (通讯作者)，Seventh Med Ctr PLA Gen Hosp, Dept Stomatol, Beijing 100700, Peoples R China.; Yi, WW (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Rehabil Med, Chongqing 400016, Peoples R China."

来源：MATERIALS & DESIGN

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001070735600001

JCR分区：Q1

影响因子：7.6

年份：2023

卷号：233

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：TiO 2 nanotubes; Osteogenic activity; Immunomodulation; Angiogenesis; Anodic oxidation

摘要："The bio-inertness of titanium (Ti) and accompanying severe inflammation can extremely inhibit the coupled osteogenesis-angiogenesis regulation and result in an undesirable osseointegration of peri-implant during the implantation process. Herein, three types of TiO2 nanotubes (TNT) were prepared on native Ti substrate using an anodic oxidation approach, including TNT with a diameter of 30 nm (TNT-30), 70 nm (TNT-70), or 110 nm (TNT-110). Among the four substrates, TNT-70 was the most prominent to induce the differentiation of macrophages to the pro-healing M2 phenotype via significant down-regulation of pro-inflammation genes (CD86, iNOS, CD11c) and cytokines (TNF-& alpha;, IL-6), as well as up-regulation of anti-inflammation genes (CD206, Arg-1, IL10) and cytokines (TGF-& beta;, IL-10). Furthermore, TNT-70 could stimulate the osteogenic potential of mesenchymal stem cells (MSCs) and the angiogenic ability of human umbilical vein endothelial cells (HUVECs) via multiple paracrine signaling. In vivo experiments suggested that TNT-70 significantly relieved early inflammatory response around the implant, facilitated coupled osteogenesis and angiogenesis, and effectively promoted the osseointegration of peri-implant. This study provided a reference for fabricating surface topography on Ti implant for improving osseointegration and relieving associated excessive inflammatory response."

基金机构："National Natural Science Foundation of China [82102537, 82101069]; Natural Science Foundation of Chongqing Science and Technology Commission [CSTC2021JCYJ-MSXMX0170, CSTB2022BSXM-JCX0039, CSTB2022 NSCQ-MSX0064, CSTB2022BSXM-JCX0058]; Beijing Municipal Science amp; Technology Commission [Z221100007422130]; Youth Incubation Program of Medical Science and Technology of PLA [21QNPY116]; First Affiliated Hospital of Chongqing Medical University cultivating fund [PYJJ2021-02, PYJJ2021-04]"

基金资助正文："This work was financially supported by National Natural Science Foundation of China (82102537 and 82101069) , Natural Science Foundation of Chongqing Science and Technology Commission (CSTC2021JCYJ-MSXMX0170, CSTB2022BSXM-JCX0039, CSTB2022 NSCQ-MSX0064, and CSTB2022BSXM-JCX0058) , the Beijing Municipal Science & Technology Commission (Z221100007422130) , the Youth Incubation Program of Medical Science and Technology of PLA (21QNPY116) , and the First Affiliated Hospital of Chongqing Medical University cultivating fund (PYJJ2021-02 and PYJJ2021-04) ."