Electric field promotes dermal fibroblast transdifferentiation through activation of RhoA/ROCK1 pathway

作者全名："Wang, Wenping; Huang, Wanqi; Liu, Jie; Zhang, Ze; Ji, Ran; Wu, Chao; Zhang, Jiaping; Jiang, Xupin"

作者地址："[Wang, Wenping; Huang, Wanqi; Liu, Jie; Zhang, Ze; Wu, Chao; Zhang, Jiaping; Jiang, Xupin] Army Med Univ, Southwest Hosp, Dept Plast Surg, State Key Lab Trauma Burns & Combined Injury, Chongqing 400038, Peoples R China; [Wang, Wenping; Ji, Ran] Chongqing Med Univ, Affiliated Hosp 1, Dept Burn & Plast Surg, Chongqing 400016, Peoples R China; [Liu, Jie] Chongqing Med Univ, Affiliated Hosp 2, Dept Plast & Maxillofacial Surg, Chongqing 400010, Peoples R China"

通信作者："Zhang, JP; Jiang, XP (通讯作者)，Army Med Univ, Southwest Hosp, Dept Plast Surg, State Key Lab Trauma Burns & Combined Injury, Chongqing 400038, Peoples R China."

来源：INTERNATIONAL JOURNAL OF MEDICAL SCIENCES

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001071182700007

JCR分区：Q1

影响因子：3.2

年份：2023

卷号：20

期号：10

开始页：1326

结束页：1335

文献类型：Article

关键词：Electric field; Myofibroblast; wound healing; chronic wound; RhoA; ROCK

摘要："With the increased incidence of age-related and lifestyle-related diseases, chronic wounds are sweeping the world, where recent studies reveal that dysfunction of fibroblast plays an indispensable role. Endogenous electric field (EF) generated by skin wound disrupting an epithelial layer has been used as an alternative clinical treatment in chronic wound by modulating cellular behaviours, including fibroblasts transdifferentiation. Although many molecules and signaling pathways have been reported associated with fibroblasts transdifferentiation, studies investigating how the electric field affects the cellular pathways have been limited. For this purpose, a model of electric field treatment in vitro was established, where cells were randomly divided into control and electrified groups. The changes of protein expression and distribution were detected under different conditions, along with Zeiss imaging system observing the response of cells. Results showed that fibroblast transdifferentiation was accompanied by increased expression of a-SMA and extracellular matrix (COL-1 and COL-3) under the EF. Simultaneously, fibroblast transdifferentiation was also consistent with changes of cell arrangement and enhanced motility. Furthermore, we found that electric field activated RhoA signaling pathways activity. Y-27632, a RhoA inhibitor, which was used to treat fibroblasts, resulted in reduced transdifferentiation. The connection between electric field and RhoA signaling pathways is likely to be significant in modulating fibroblast transdifferentiation in acute injury and tissue remodeling, which provides an innovative idea for the molecular mechanism of EF in promoting chronic wound healing."

基金机构："Science and Technology Innovation Enhancement Project of Army Medical University [2020XQN12]; General program of Natural Science Foundation of Chongqing [CSTB2023NSCQ-MSX0184, SZYZ-TR-14]; Scientific Research Project of Chongqing [CSTB2022BSXM-JCX0010]"

基金资助正文："This research was funded by Science and Technology Innovation Enhancement Project of Army Medical University (2020XQN12), General program of Natural Science Foundation of Chongqing (CSTB2023NSCQ-MSX0184), Growth factor Recovery Program (SZYZ-TR-14), and Scientific Research Project of Chongqing (CSTB2022BSXM-JCX0010)."