A Highly Sensitive Electrochemical Sensor for Capsaicinoids and Its Application in the Identification of Illegal Cooking Oil

作者全名："Lyu, Wenjing; Ding, Min; Zhou, Ying; Jiang, Mengdan; Li, Yanru; Ding, Yanxiang; Zhang, Zhong; Wei, Xue; Zhang, Xiaoqing"

作者地址："[Lyu, Wenjing; Ding, Min; Zhou, Ying; Jiang, Mengdan; Li, Yanru; Wei, Xue; Zhang, Xiaoqing] Chongqing Med Univ, Sch Lab Med, Key Lab Clin Lab Diagnost, Minist Educ China, Chongqing 400016, Peoples R China; [Ding, Yanxiang] Chongqing Med Univ, Clin Coll 1, Chongqing 400016, Peoples R China; [Zhang, Zhong] Chongqing Publ Secur Bur, Mat Evidence Identificat Ctr, Chongqing 400016, Peoples R China"

通信作者："Zhang, XQ (通讯作者)，Chongqing Med Univ, Sch Lab Med, Key Lab Clin Lab Diagnost, Minist Educ China, Chongqing 400016, Peoples R China."

来源：BIOSENSORS-BASEL

ESI学科分类：　

WOS号：WOS:001071296600001

JCR分区：Q1

影响因子：4.9

年份：2023

卷号：13

期号：9

开始页：　

结束页：　

文献类型：Article

关键词：capsaicinoids; illegal cooking oil; screen-printed carbon electrode; composite nanoparticles; diazotization-coupling reaction

摘要："Capsaicinoids, mostly from chili peppers, are widely used in daily life. Capsaicinoids are considered to be markers for the identification of illegal cooking oil (ICO), which is a serious threat to public health. The identification of capsaicinoids can help reveal food-related fraud, thereby safeguarding consumers' health. Here, a novel and ultrasensitive method was established with a signal amplification strategy for the detection of capsaicinoids. AuNPs@Fe3O4 nanocomposites were functionalized with 4-aminothiophenol (4-atp). After diazotization, 4-atp on AuNPs@Fe3O4 reacted with capsaicinoids and formed capsaicinoids-azo-atp-AuNPs@Fe3O4. Ultimately, capsaicinoids-azo-atp-AuNPs@Fe3O4 was dropped onto the surface of a screen-printed carbon electrode (SPCE) and detected via the differential pulse voltammetry (DPV) method. AuNPs@Fe3O4 nanocomposites increased the specific surface area of the electrode. Moreover, the diazotization-coupling reaction enriched the analytes on the electrode surface. Liquid-liquid extraction was used for sample pretreatment. Under a pH value of 9.0 and concentration of 0.20 mol/L for the supporting electrolyte, the linearity of capsaicinoids in ICO is from 0.10 to 10.00 ng/mL, and the limit of detection (S/N = 3) is 0.05 ng/mL. This method is ultra-sensitive, reliable, and cost-effective for the detection of capsaicinoids. Herein, this method provides a promising tool for the identification of ICO."

基金机构：We earnestly thank the Chongqing Municipal Material Evidence Identification Center for providing ICO samples.

基金资助正文：We earnestly thank the Chongqing Municipal Material Evidence Identification Center for providing ICO samples.