Recent Advances and Innovations in the Preparation and Purification of In Vitro-Transcribed-mRNA-Based Molecules

作者全名："Zhang, Jingjing; Liu, Yuheng; Li, Chao; Xiao, Qin; Zhang, Dandan; Chen, Yang; Rosenecker, Joseph; Ding, Xiaoyan; Guan, Shan"

作者地址："[Zhang, Jingjing; Liu, Yuheng; Li, Chao; Xiao, Qin; Zhang, Dandan; Chen, Yang; Guan, Shan] Third Mil Med Univ, Natl Engn Res Ctr Immunol Prod, Chongqing 400038, Peoples R China; [Liu, Yuheng] Chongqing Med Univ, Coll Pharm, Dept Pharmacol, Chongqing 400016, Peoples R China; [Rosenecker, Joseph; Ding, Xiaoyan] Ludwig Maximilians Univ Munchen, Dept Pediat, D-80337 Munich, Germany"

通信作者："Guan, S (通讯作者)，Third Mil Med Univ, Natl Engn Res Ctr Immunol Prod, Chongqing 400038, Peoples R China.; Ding, XY (通讯作者)，Ludwig Maximilians Univ Munchen, Dept Pediat, D-80337 Munich, Germany."

来源：PHARMACEUTICS

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:001072302200001

JCR分区：Q1

影响因子：4.9

年份：2023

卷号：15

期号：9

开始页：　

结束页：　

文献类型：Review

关键词：IVT mRNA; vaccine; purification strategy; sequence design; SARS-CoV-2

摘要："The coronavirus disease 2019 (COVID-19) pandemic poses a disruptive impact on public health and the global economy. Fortunately, the development of COVID-19 vaccines based on in vitro-transcribed messenger RNA (IVT mRNA) has been a breakthrough in medical history, benefiting billions of people with its high effectiveness, safety profile, and ease of large-scale production. This success is the result of decades of continuous RNA research, which has led to significant improvements in the stability and expression level of IVT mRNA through various approaches such as sequence optimization and improved preparation processes. IVT mRNA sequence optimization has been shown to have a positive effect on enhancing the mRNA expression level. The innovation of IVT mRNA purification technology is also indispensable, as the purity of IVT mRNA directly affects the success of downstream vaccine preparation processes and the potential for inducing unwanted side effects in therapeutic applications. Despite the progress made, challenges related to IVT mRNA sequence design and purification still require further attention to enhance the quality of IVT mRNA in the future. In this review, we discuss the latest innovative progress in IVT mRNA design and purification to further improve its clinical efficacy."

基金机构："We would like to thank the Laboratory of Materials Engineering at the University of Applied Sciences Muenster, headed by Gerhard Gevelmann and Miriam Laubrock, for the use of the SEM, including the EDS-Analysis and the support in the analysis of the result"

基金资助正文："We would like to thank the Laboratory of Materials Engineering at the University of Applied Sciences Muenster, headed by Gerhard Gevelmann and Miriam Laubrock, for the use of the SEM, including the EDS-Analysis and the support in the analysis of the results."