"LINC01480 as a Prognostic Biomarker Associated With Proliferation, Migration, and Invasion of Clear Cell Renal Cell Carcinoma"

作者全名："Ma, Yan; Wu, Chunyang; Deng, Shixiong"

作者地址："[Ma, Yan; Deng, Shixiong] Chongqing Med Univ, Coll Basic Med, Lab Forens Med & Biomed Informat, Chongqing, Peoples R China; [Ma, Yan] Chongqing Med Univ, Informat Ctr Chongqing Med Univ, Chongqing, Peoples R China; [Ma, Yan] Chongqing Med Univ, Coll Biomed Engn, Chongqing, Peoples R China; [Wu, Chunyang] Chongqing Shapingba Hosp Chinese Med, Chongqing, Peoples R China"

通信作者："Deng, SX (通讯作者)，Chongqing Med Univ, Lab Forens Med & Biomed Informat, Coll Basic Med, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China."

来源：ANTICANCER RESEARCH

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001072909600016

JCR分区：Q4

影响因子：1.6

年份：2023

卷号：43

期号：7

开始页：3037

结束页：3046

文献类型：Article

关键词：LINC01480; clear cell renal cell carcinoma; prognosis; cancer metastasis

摘要："Background/ Aim: The present study aimed to identify key long noncoding RNAs ( lncRNAs) involved in survival and metastasis of clear cell renal cell carcinoma (ccRCC). Materials and Methods: A systemic screening for genes with differential expression in ccRCC was performed using publicly available databases. Cox regression analysis was used to identify lncRNAs associated with survival. A competing endogenous RNAs (ceRNA) regulation network of metastasis-related lncRNAs was constructed and hub lncRNAs were identified. Functional and pathway enrichment analyses were performed to investigate the role of lncRNA in ccRCC. Cell Counting Kit- 8 and Transwell assays were used to determine the levels of cell proliferation, migration, and invasion. Results: A total of 732 lncRNAs were found to be differentially expressed between ccRCC tumors and healthy samples. Among them, 139 lncRNAs were differentially expressed between metastasis and non- metastasis ccRCC samples and 75 lncRNAs were associated with overall survival and curated metastasis-related genes. Notably, LINC01480 was identified as the hub lncRNA involved in regulation of ccRCC metastasis. Clinically, LINC01480 may act as an independent factor for poor overall survival of ccRCC patients (log-rank p<0.05). Reverse transcription-quantitative PCR analysis validated that LINC01480 was significantly up-regulated in ccRCC compared to paired normal samples (n=20). Moreover, LINC01480 silencing inhibited the proliferation, migration, and invasion of ccRCC cells in vitro. Gene set enrichment analysis showed that high LINC01480 expression may promote ccRCC metastasis through enhancing immunodeficiency and amino acid metabolism. Conclusion: LINC01480 may act as a novel biomarker for overall prognosis in ccRCC and exhibit potential as a therapeutic target for the treatment of metastatic ccRCC."

基金机构：Intelligent Medicine Research Project of Chongqing Medical University [ZHYX202231]

基金资助正文：The present study was funded by the Intelligent Medicine Research Project of Chongqing Medical University (grant no. ZHYX202231).