Single-cell transcriptome sequencing reveals tumor heterogeneity in family neuroblastoma

作者全名："Zhang, Yunlong; Ma, Yue; Liu, Qingqing; Du, Yifei; Peng, Liang; Zhou, Jianwu; Zhao, Zhenzhen; Li, Changchun; Wang, Shan"

作者地址："[Zhang, Yunlong; Ma, Yue; Liu, Qingqing; Du, Yifei; Peng, Liang; Zhou, Jianwu; Zhao, Zhenzhen; Li, Changchun; Wang, Shan] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Minist Educ,Key Lab Child Dev & Disorders, Chongqing Key Lab Pediat,Dept Pediat Surg Oncol,Ch, Chongqing, Peoples R China"

通信作者："Wang, S (通讯作者)，Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Minist Educ,Key Lab Child Dev & Disorders, Chongqing Key Lab Pediat,Dept Pediat Surg Oncol,Ch, Chongqing, Peoples R China."

来源：FRONTIERS IN IMMUNOLOGY

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001074263900001

JCR分区：Q1

影响因子：5.7

年份：2023

卷号：14

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：family neuroblastoma (FNB); single-cell RNA sequencing (scRNA-seq); single-cell flux estimation analysis (scFEA); SDHD; cancer-associated fibroblasts (CAF); tumor microenvironment

摘要："Neuroblastoma(NB) is the most common extracranial solid tumor in childhood, and it is now believed that some patients with NB have an underlying genetic susceptibility, which may be one of the reasons for the multiplicity of NB patients within a family line. Even within the same family, the samples show great variation and can present as ganglioneuroblastoma or even benign ganglioneuroma. The genomics of NB is still unclear and more in-depth studies are needed to reveal its key components. We first performed single-cell RNA sequencing(sc-RNAseq) analysis on clinical specimens of two family neuroblastoma(FNB) and four sporadic NB cases. A complete transcriptional profile of FNB was constructed from 18,394 cells from FNB, and we found that SDHD may be genetically associated with FNB and identified a prognostic related CAF subtype in FNB: Fib-4. Single-cell flux estimation analysis (scFEA) results showed that malignant cells were associated with arginine spermine, oxaloacetate and hypoxanthine, and that malignant cells metabolize lactate at lower levels than T cells. Our study provides new resources and ideas for the development of the genomics of family NB, and the mechanisms of cell-to-cell interactions and communication and the metabolic landscape will provide new therapeutic targets."

基金机构："This work was supported in part by research grants from the Key Project of ""Research on Prevention and Control of Major Chronic Non-Communicable Diseases"", the Ministry of Science and Technology of the People's Republic of China, National Key Ramp;amp;D P; Key Project of ""Research on Prevention and Control of Major Chronic Non-Communicable Diseases""; Ministry of Science and Technology of the People's Republic of China [2018YFC1313000, 2018YFC1313004]; National Key Ramp;amp;D Program of China [YBXM-2019-003]; General Clinical Medical Research Program of Children's Hospital of Chongqing Medical University"

基金资助正文："This work was supported in part by research grants from the Key Project of ""Research on Prevention and Control of Major Chronic Non-Communicable Diseases"", the Ministry of Science and Technology of the People's Republic of China, National Key R & amp;D Program of China (No. 2018YFC1313000, 2018YFC1313004), the General Clinical Medical Research Program of Children's Hospital of Chongqing Medical University (No. YBXM-2019-003)."