"A new sandwich-type electrochemiluminescence sensor based on HPSNs-NH2@Au NPs and AuPdPt NPs for determination of α(2,3)-sial-Gs"

作者全名："Tian, Jiangman; Zhu, Qihao; Huang, Xiaojing; Li, Yuan"

作者地址："[Tian, Jiangman] Chongqing Med Univ, Yongchuan Hosp, Dept Pharm, Chongqing 402160, Peoples R China; [Zhu, Qihao] Yongchuan Hosp Tradit Chinese Med, Dept Pharm, Chongqing 402160, Peoples R China; [Huang, Xiaojing; Li, Yuan] Chongqing Med Univ, Yongchuan Hosp, Cent Lab, Chongqing 402160, Peoples R China"

通信作者："Li, Y (通讯作者)，Chongqing Med Univ, Yongchuan Hosp, Cent Lab, Chongqing 402160, Peoples R China."

来源：MICROCHIMICA ACTA

ESI学科分类：CHEMISTRY

WOS号：WOS:001075681100005

JCR分区：Q1

影响因子：5.3

年份：2023

卷号：190

期号：10

开始页：　

结束页：　

文献类型：Article

关键词：Electrochemiluminescence; Surface modification; Amino-functionalized porous silica nanoparticles; AuPdPt trimetallic nanoparticles; Signal quenching

摘要："A novel sandwich-type ""on-off"" electrochemiluminescence (ECL) biosensor for the determination of alpha(2,3)-sial-Gs was designed. Specifically, amino-functionalized porous silica nanoparticles (HPSNs-NH2) were first prepared and then decorated with gold nanoparticles (Au NPs) to form HPSNs-NH2@Au NP nanocomposite, which exhibited a strong ability to enhance ECL intensity with K2S2O8 as co-reactant (signal-on) and could immobilize the target-specific binding molecules of maackia amurensis lectin (MAL). Additionally, AuPdPt trimetallic nanoparticles were prepared to serve as a quenched ECL signal indicator (signal-off) with the ability of capturing the target non-specific binding molecules of 3-aminophenylboronic acid (APBA) to form a signal label. The sandwich-type ECL biosensor was constructed based on the structure of MAL-alpha(2,3)-sial-Gs-APBA and achieved a determination toward alpha(2,3)-sial-Gs with a wide linear range from 1 fg mL-1 to 10 ng mL-1 and a low detection limit of 0.5 fg mL-1. Furthermore, the proposed ECL biosensor showed satisfactory selectivity, stability, and reproducibility for alpha(2,3)-sial-Gs determination."

基金机构："This work is supported by Medical Research Program of Chongqing Municipal Health Commission (2023WSJK101), National Natural Science Foundation of China (11702047), Technology Innovation Special Program of Social Undertaking and Livelihood Security Science [2023WSJK101]; Medical Research Program of Chongqing Municipal Health Commission [11702047]; National Natural Science Foundation of China [cstc2017shmsA130009]; Technology Innovation Special Program of Social Undertaking and Livelihood Security Science of Chongqing [2017MSXM079]; Medical Research Program of Chongqing [Ycstc2018cc0206]; Social Livelihood and Technology Innovation Special program of Yongchuan, Chongqing"

基金资助正文："This work is supported by Medical Research Program of Chongqing Municipal Health Commission (2023WSJK101), National Natural Science Foundation of China (11702047), Technology Innovation Special Program of Social Undertaking and Livelihood Security Science of Chongqing (cstc2017shmsA130009), Medical Research Program of Chongqing (2017MSXM079), and Social Livelihood and Technology Innovation Special program of Yongchuan, Chongqing (Ycstc2018cc0206)."