Function and autophagy of monocyte-derived dendritic cells is affected by hepatitis B virus infection

作者全名："Xu, Hua; Kang, Juan; Zhong, Shan; Chen, Min; Hu, Peng; Ren, Hong; Zhou, Zhi; Lei, Yu"

作者地址："[Xu, Hua; Kang, Juan; Zhong, Shan; Chen, Min; Hu, Peng; Ren, Hong; Zhou, Zhi; Lei, Yu] Chongqing Med Univ, Affiliated Hosp 2, Inst Viral Hepatitis, Dept Infect Dis,Minist Educ,Key Lab Mol Biol Infec, 288 Tianwen Rd,Nan Ping Dist, Chongqing 400060, Peoples R China; [Xu, Hua] Chongqing Hosp Tradit Chinese Med, Dept Oncol, 6,7 Branch Rd,Panxi,Jiangbei Dist, Chongqing 400021, Peoples R China"

通信作者："Lei, Y (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Inst Viral Hepatitis, Dept Infect Dis,Minist Educ,Key Lab Mol Biol Infec, 288 Tianwen Rd,Nan Ping Dist, Chongqing 400060, Peoples R China."

来源：BMC IMMUNOLOGY

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001075952200001

JCR分区：Q3

影响因子：2.9

年份：2023

卷号：24

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Hepatitis B virus; Monocyte-derived dendritic cell; Phenotype; Cytokine; Autophagy

摘要："BackgroundThe role of dendritic cells and the autophagy state of dendritic cells in the immune response of hepatitis B virus (HBV) infection was still controversial. In this study, we carefully examined the phenotype, function and autophagy pathway of dendritic cells in HBV infection.MethodsMonocyte-derived dendritic cells from healthy blood donors and patients with chronic HBV infection were stimulated by lipopolysaccharide, supernatant of HepG2.2.15 cells or supernatant of HepG2 cells respectively. Phenotype of dendritic cells was examined by flow cytometry and cytokines secretion was detected by enzyme-linked immunosorbent assay. Autophagy related proteins were detected by western blot and immunofluorescence analysis.ResultsOur results showed that the expression of both major histocompatibility complex II molecules and co-stimulated molecules including cluster of differentiation antigen 80, cluster of differentiation antigen 86 in the monocyte-derived dendritic cells from patients with chronic HBV infection was significantly higher than that from healthy donors when cultured with supernatant of HepG2.2.15 cells. The amount of cytokines, including tumour necrosis factor-alpha, interleukin-10 and interleukin-12, secreted by monocyte-derived dendritic cells from patients with chronic HBV infection was also significantly higher than that from healthy donors when stimulate by HBV. Interestingly, the expression level of autophagy-related proteins including autophagy-related protein5 and associated protein 1 light chain in dendritic cells from patients with chronic HBV infection was significantly increased when compared with that from healthy donors when re-exposed to HBV.ConclusionsOur results indicated that dendritic cells from patients with chronic HBV infection could intensively present antigen and express co-stimulatory molecules. The increased activation of dendritic cells might be related to the enhanced autophagy of dendritic cells in HBV infection."

基金机构：We thank all the patients or healthy donors enrolled in this study for their kindly understanding and supporting. We also thank the faculty of the department of infectious disease who contribute to subjects screening and blood sample collection.

基金资助正文：We thank all the patients or healthy donors enrolled in this study for their kindly understanding and supporting. We also thank the faculty of the department of infectious disease who contribute to subjects screening and blood sample collection.