Development and Validation of a Novel Prognosis Model Based on a Panel of Three Immunogenic Cell Death-Related Genes for Non-Cirrhotic Hepatocellular Carcinoma

作者全名："Gong, Jiaojiao; Yu, Renjie; Hu, Xiaoxia; Luo, Huating; Gao, Qingzhu; Li, Yadi; Tan, Guili; Luo, Haiying; Qin, Bo"

作者地址："[Gong, Jiaojiao; Yu, Renjie; Hu, Xiaoxia; Luo, Huating; Li, Yadi; Tan, Guili; Luo, Haiying; Qin, Bo] Chongqing Med Univ, Affiliated Hosp 1, Dept Infect Dis, Chongqing Key Lab Infect Dis & Parasit Dis, 1 Youyi Rd, Chongqing 400016, Peoples R China; [Gong, Jiaojiao] Chongqing Med Univ, Dept Nephrol, Bishan Hosp, Chongqing, Peoples R China; [Gao, Qingzhu] Chongqing Med Univ, Affiliated Hosp 2, Dept Infect Dis, Chongqing, Peoples R China"

通信作者："Qin, B (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Infect Dis, Chongqing Key Lab Infect Dis & Parasit Dis, 1 Youyi Rd, Chongqing 400016, Peoples R China."

来源：JOURNAL OF HEPATOCELLULAR CARCINOMA

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001076142600001

JCR分区：Q2

影响因子：4.2

年份：2023

卷号：10

期号：　

开始页：1609

结束页：1628

文献类型：Article

关键词：immunogenic cell death-related genes; non-cirrhotic hepatocellular carcinoma; risk stratification; nomogram

摘要："Purpose: The accurate prediction of non-cirrhotic hepatocellular carcinoma (NCHCC) risk facilitates improved surveillance strategy and decreases cancer-related mortality. This study aimed to explore the correlation between immunogenic cell death (ICD) and NCHCC prognosis using The Cancer Genome Atlas (TCGA) datasets, and the potential prognostic value of ICD-related genes in NCHCC.Methods: Clinical and transcriptomic data of patients with NCHCC patients were retrieved from TCGA database. Weighted gene co -expression network analysis was performed to obtain the NCHCC phenotype-related module genes. Consensus clustering analysis was performed to classify the patients into two clusters based on intersection genes among differentially expressed genes (DEGs) between cancer and adjacent tissues, NCHCC phenotype-related genes, and ICD-related genes. NCHCC-derived tissue microarray was used to evaluate the correlation of the expression levels of key genes with NCHCC prognosis using immunohistochemical staining.Results: Cox regression analyses were performed to construct a prognostic risk score model comprising three genes (TMC7, GRAMD1C, and GNPDA1) based on DEGs between two clusters. The model stratified patients with NCHCC into two risk groups. The overall survival (OS) of the high-risk group was significantly lower than that of the low-risk group. Univariable and multivariable Cox regression analyses revealed that these signature genes are independent predictors of OS. Functional analysis revealed differential immune status between the two risk groups. Next, a nomogram was constructed, which demonstrated the potent distinguishing ability of the developed model based on receiver operating characteristic curves. In vitro functional validation revealed that the migration and invasion abilities of HepG2 and Huh7 cells were upregulated upon GRAMD1C knockdown but downregulated upon TMC7 knockdown.Conclusion: This study developed a prognostic model comprising three genes, which can aid in predicting the survival of patients with NCHCC and guide the selection of drugs and molecular markers for NCHCC."

基金机构：General Project of Chongqing Natural Science Foundation of China [03010102 2020MSXM026]

基金资助正文：Funding This study was supported by the General Project of Chongqing Natural Science Foundation of China (03010102 2020MSXM026) .