The Impact of ESBLs-Positive<i> Escherichia</i><i> coli's</i> Resistance to Cefepime and Its Guidance for Clinical Treatment

作者全名："Wu, Qian; Shi, Jing; Huang, Juan; Gan, Delu; Zhang, Lijun; Li, Pu"

作者地址："[Wu, Qian; Gan, Delu; Zhang, Lijun; Li, Pu] Chongqing Med Univ, Hosp 2, Dept Lab Med, Chongqing, Peoples R China; [Shi, Jing] Chongqing Med Univ, Affiliated Hosp 1, Dept Clin Lab, Chongqing, Peoples R China; [Huang, Juan] Chongqing Med Univ, Affiliated Hosp 2, Dept Informat Ctr, Chongqing, Peoples R China; [Li, Pu] Chongqing Med Univ, Affiliated Hosp 2, Dept Clin Lab, Chongqing, Peoples R China"

通信作者："Li, P (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Clin Lab, Chongqing, Peoples R China."

来源：INFECTION AND DRUG RESISTANCE

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001076948100001

JCR分区：Q2

影响因子：2.9

年份：2023

卷号：16

期号：　

开始页：6395

结束页：6404

文献类型：Article

关键词：Escherichia coli; cefepime; ESBLs; mutant prevention concentration

摘要："Background: Escherichia coli (E. coli) is a common pathogen in bloodstream infections (BSI), and the production of extended-spectrum beta-lactamases (ESBLs) is its main mechanism of resistance. However, the impact of different ESBL genotypes of E. coli on the resistance to Cefepime (FEP) remains unclear.Methods: A total of 2356 cases of BSI patients were collected. The experimental group included 188 ESBL-positive E. coli strains that were resistant to FEP but sensitive to ceftazidime (CAZ). Antibiotic usage and resistance rates were evaluated through antimicrobial susceptibility testing and antibiotic usage records. The ESBL genotypes were identified, and the minimum inhibitory concentration (MIC) and mutant prevention concentration (MPC) of FEP were determined.Results: In ESBL-positive E. coli, three ESBL genotypes were identified: 188 strains of CTX-M, 130 strains of TEM-1, and 26 strains of OXA-10. Among them, 124 strains carried both CTX-M-9 and TEM-1 genotypes, 22 strains carried two CTX-M genotypes (CTX-M-1 and CTX-M-2), 20 strains carried both CTX-M-9 and OXA-10, and 6 strains carried three genotypes (CTX-M-9, CTX-TEM-1, and OXA-10). The MIC50, MIC90, MPC50, and MPC90 of the 188 ESBL-positive E. coli were 64, 256, 128, and 528, respectively. The MIC values ranged from 32 to 256, while the MPC values ranged from 64 to 528. The MIC50, MIC90, MPC50, and MPC90 of the 40 ESBL-negative E. coli were 0.5, 1, 64, and 128, respectively; the MIC values ranged from 0.25 to 4, while the MPC values ranged from 32 to 256, respectively. Conclusion: ESBL-positive E. coli induces an increase in the MIC value of FEP, leading to an increase in FEP resistance. The inoculation effect also causes a significant increase in the MPC value of FEP, especially the increase in selection index value, indicating selective enrichment and amplification of drug-resistant mutants, resulting in clinical treatment failure."

基金机构：Joint general project of the Chongqing Science and Technology Bureau; Chongqing Municipal Health Commission [2023MSXM117]

基金资助正文：Funding This work was supported by the Joint general project of the Chongqing Science and Technology Bureau and the Chongqing Municipal Health Commission (2023MSXM117) .