Rapid Evaluation of Antibacterial Carbohydrates on a Microfluidic Chip Integrated with the Impedimetric Neoglycoprotein Biosensor

作者全名："Ji, Haijie; Yang, Xueqiong; Zhou, Hang; Cui, Feiyun; Zhou, Qin"

作者地址："[Ji, Haijie; Yang, Xueqiong; Zhou, Hang; Cui, Feiyun; Zhou, Qin] Chongqing Med Univ, Sch Lab Med, Minist Educ Key Lab Clin Diagnost, Chongqing 400016, Peoples R China"

通信作者："Cui, FY; Zhou, Q (通讯作者)，Chongqing Med Univ, Sch Lab Med, Minist Educ Key Lab Clin Diagnost, Chongqing 400016, Peoples R China."

来源：BIOSENSORS-BASEL

ESI学科分类：　

WOS号：WOS:001077169600001

JCR分区：Q1

影响因子：4.9

年份：2023

卷号：13

期号：9

开始页：　

结束页：　

文献类型：Article

关键词：impedimetric biosensor; microfluidic chip; glycoprotein nano-sensing surface; Man-BSA/Au NPs; antiadhesive carbohydrates

摘要："The colonization of some bacteria to their host cell is mediated by selective adhesion between adhesin and glycan. The evaluation of antiadhesive carbohydrates in vitro has great significance in discovering new antibacterial drugs. In this paper, a microfluidic chip integrated with impedimetric neoglycoprotein biosensors was developed to evaluate the antibacterial effect of carbohydrates. Mannosylated bovine serum albumin (Man-BSA) was taken as the neoglycoprotein and immobilized on the microelectrode-modified gold nanoparticles (Au NPs) to form a bionic glycoprotein nanosensing surface (Man-BSA/Au NPs). Salmonella typhimurium (S. typhimurium) was selected as a bacteria model owing to its selective adhesion to the mannose. Electrochemical impedance spectroscopy (EIS) was used to characterize the adhesion capacity of S. typhimurium to the Man-BSA/Au NPs and evaluate the antiadhesive efficacy of nine different carbohydrates. It was illustrated that the 4-methoxyphenyl-alpha-D-pyran mannoside (Phenyl-Man) and mannan peptide (Mannatide) showed excellent antiadhesive efficacy, with IC50 values of 0.086 mM and 0.094 mM, respectively. The microfluidic device developed in this study can be tested in multiple channels. Compared with traditional methods for evaluating the antibacterial drug in vitro, it has the advantages of being fast, convenient, and cost-effective."

基金机构："National Natural Science Funds of China [82102502, 82030065]"

基金资助正文："This research was funded by the National Natural Science Funds of China, grant numbers 82102502 and 82030065."