rFSAV promotes Staphylococcus aureus-infected bone defect healing via IL-13-mediated M2 macrophage polarization

作者全名："Zhang, Yanhao; Yang, Feng; Sun, Dong; Xu, Limin; Shi, Yaojia; Qin, Leilei; Zhao, Liqun; Wang, Liting; Sun, Wei; Wu, Hongri; Lu, Dongshui; Zhang, Weijun; Luo, Ping; Cheng, Ping; Zou, Quanming; Zeng, Hao"

作者地址："[Zhang, Yanhao; Shi, Yaojia; Zhao, Liqun; Lu, Dongshui; Zhang, Weijun; Luo, Ping; Cheng, Ping; Zou, Quanming; Zeng, Hao] Third Mil Med Univ, Coll Pharm, Natl Engn Res Ctr Immunol Prod, Dept Microbiol & Biochem Pharm, Chongqing 400038, Peoples R China; [Yang, Feng; Xu, Limin] Chengdu Olymvax Biopharmaceut Inc, Chengdu 611731, Sichuan, Peoples R China; [Sun, Dong; Wu, Hongri] Third Mil Med Univ, Southwest Hosp, Dept Orthoped, Chongqing 400038, Peoples R China; [Qin, Leilei] Chongqing Med Univ, Affiliated Hosp 1, Dept Orthoped, Chongqing 400038, Peoples R China; [Wang, Liting; Sun, Wei] Third Mil Med Univ, Biomed Anal Ctr, Chongqing 400038, Peoples R China; [Zeng, Hao] Third Mil Med Univ, State Key Lab Trauma & Chem Poisoning, Chongqing, Peoples R China"

通信作者："Zou, QM; Zeng, H (通讯作者)，Third Mil Med Univ, Coll Pharm, Natl Engn Res Ctr Immunol Prod, Dept Microbiol & Biochem Pharm, Chongqing 400038, Peoples R China.; Zeng, H (通讯作者)，Third Mil Med Univ, State Key Lab Trauma & Chem Poisoning, Chongqing, Peoples R China."

来源：CLINICAL IMMUNOLOGY

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001077266200001

JCR分区：Q2

影响因子：4.5

年份：2023

卷号：255

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Bone infection; Interleukin-13; Macrophage polarization; Staphylococcus aureus; Vaccine

摘要："Staphylococcus aureus (S. aureus) contamination commonly occurs in orthopedic internal fixation operations, leading to a delayed healing of the defected bone tissue. However, antibiotic treatments are ineffective in dealing with S. aureus bone infections due to the rise in multiple antimicrobial resistances. Here, we reported the protective effects of a recombinant five-antigen S. aureus vaccine (rFSAV) in an S. aureus infected bone defect model. In this study, we found the number of M2 macrophages markedly increased in the defect site and played a critical role in the healing of defected bone mediated by rFSAV. Mechanistically, rFSAV mediated increased level of IL13 in bone defect site predominant M2 macrophage polarization. In summary, our study reveals a key role of M2 macrophage polarization in the bone regeneration process in S. aureus infection induced bone defect, which provide a promising application of rFSAV for the treatment of bone infection for orthopedic applications."

基金机构：National Key Research and Devel-opment Program of China [2021YFC2301405]; National Natural Science Foundation of China [32070941]; Technology Innovation and application development project of Chongqing [CQYC202003220]

基金资助正文："This work was supported by the National Key Research and Devel-opment Program of China (2021YFC2301405) , National Natural Science Foundation of China (32070941) , and Technology Innovation and application development project of Chongqing (CQYC202003220) ."