"HISTOLOGY, DIMENSIONS, AND FLUORESCEIN STAINING CHARACTERISTICS OF NODULAR AND CUTICULAR DRUSEN IN AGE-RELATED MACULAR DEGENERATION"

作者全名："Evers III, Charles D.; Chen, Ling; Messinger, Jeffrey D.; Killingsworth, Murray; Freund, K. Bailey; Curcio, Christine A."

作者地址："[Evers III, Charles D.; Chen, Ling; Messinger, Jeffrey D.; Curcio, Christine A.] Univ Alabama Birmingham, Heersink Sch Med, Dept Ophthalmol & Visual Sci, Birmingham, AL 35294 USA; [Chen, Ling] Chongqing Med Univ, Affiliated Hosp 1, Chongqing Key Lab Ophthalmol, Chongqing, Peoples R China; [Chen, Ling] Chongqing Eye Inst, Chongqing, Peoples R China; [Killingsworth, Murray] Western Sydney Univ, Sch Med, Discipline Pathol, Sydney, NSW, Australia; [Killingsworth, Murray] Univ New South Wales, Fac Med, South West Sydney Clin Campus, Sydney, NSW, Australia; [Killingsworth, Murray] NSW Hlth Pathol & Ingham Inst Appl Med Res, Liverpool, NSW, Australia; [Freund, K. Bailey] Vitreous Retina Macula Consultants New York, New York, NY USA; [Freund, K. Bailey] Grossman New York Univ, Sch Med, Dept Ophthalmol, New York, NY USA; [Curcio, Christine A.] Univ Alabama Birmingham, Alabama Vis Res Labs, Heersink Sch Med, Dept Ophthalmol & Visual Sci,EyeSight Fdn, 1670 Univ Blvd Room 360, Birmingham, AL 35294 USA"

通信作者："Curcio, CA (通讯作者)，Univ Alabama Birmingham, Alabama Vis Res Labs, Heersink Sch Med, Dept Ophthalmol & Visual Sci,EyeSight Fdn, 1670 Univ Blvd Room 360, Birmingham, AL 35294 USA."

来源：RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001077857400010

JCR分区：Q2

影响因子：2.3

年份：2023

卷号：43

期号：10

开始页：1708

结束页：1716

文献类型：Article

关键词：age-related macular degeneration; drusen; fluorescein angiography; retinal pigment epithelium; histology; electron microscopy; optical coherence tomography

摘要："Purpose: To enable in vivo analysis of drusen composition and lifecycle, the macular nodular and cuticular drusen were assessed using histology.Methods: Median and interquartile range of base widths of single (nonconfluent) nodular drusen in three sources were determined histologically: 43 eyes of 43 clinically undocumented donors, in an online resource; one eye with punctate hyperfluorescence in fluorescein angiography; and two eyes of one patient with bilateral ""starry sky"" cuticular drusen. All tissues were processed for high-resolution epoxy-resin histology and for cuticular drusen, transmission electron microscopy.Results: All drusen localized between the retinal pigment epithelium basal lamina and inner collagenous layer of the Bruch membrane. They were solid, globular, homogeneously stained with toluidine blue, and uncovered by basal laminar deposit and basal mounds. Median base widths were 13.0 mu m (Source 1, N = 128 drusen, interquartile range 7.7, 20.0 mu m), 15.3 mu m (Source 2, N = 87, interquartile range 10.6, 20.5 mu m), and 7.3 mu m (Source 3, N = 78, interquartile range 3.9, 14.1 mu m).Conclusion: In three samples, >90% of solitary nodular drusen were <30 mu m, the visibility threshold in color fundus photography; these drusen are hyperfluorescent in fluorescein angiography. Whether these progress to soft drusen, known as high-risk from epidemiology studies and hypofluorescent, may be determinable from multimodal imaging datasets that include fluorescein angiography."

基金机构：The authors thank Advancing Sight Network for timely retrieval of donor eyes.

基金资助正文：The authors thank Advancing Sight Network for timely retrieval of donor eyes.