Hypoxia improves self-renew and migration of urine-derived stem cells by upregulating autophagy and mitochondrial function through ERK signal pathway

作者全名："Hu, Chaoqun; Sun, Yanting; Li, Wanxia; Bi, Yang"

作者地址："[Hu, Chaoqun; Sun, Yanting; Li, Wanxia; Bi, Yang] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders Chongqing, Stem Cell Biol & Therapy Lab, Minist Educ,Key Lab Child Dev & Disorders,Children, Bldg 7,Room 905,136 Zhongshan Er Rd, Chongqing 400014, Peoples R China; [Hu, Chaoqun] Chongqing Peoples Hosp, Digest Dept, Chongqing, Peoples R China; [Sun, Yanting] Soochow Univ, Affiliated Hosp 1, Ctr Clin Lab, Suzhou 215006, Peoples R China; [Bi, Yang] Chongqing Med Univ, Stem Cell Biol & Therapy Lab, Childrens Hosp, Bldg 7,Room 905,136 Zhongshan Er Rd, Chongqing 400014, Peoples R China"

通信作者："Bi, Y (通讯作者)，Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders Chongqing, Stem Cell Biol & Therapy Lab, Minist Educ,Key Lab Child Dev & Disorders,Children, Bldg 7,Room 905,136 Zhongshan Er Rd, Chongqing 400014, Peoples R China.; Bi, Y (通讯作者)，Chongqing Med Univ, Stem Cell Biol & Therapy Lab, Childrens Hosp, Bldg 7,Room 905,136 Zhongshan Er Rd, Chongqing 400014, Peoples R China."

来源：MITOCHONDRION

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001078258400001

JCR分区：Q1

影响因子：3.9

年份：2023

卷号：73

期号：　

开始页：1

结束页：9

文献类型：Article

关键词：Urine-derived stem cells; ERK signal pathway; Hypoxia; Autophagy; Mitochondrial function

摘要："Urine-derived stem cells (USCs) are autologous stem cells with self-renewal ability and multi-lineage differentiation potential. Our previous studies have shown that hypoxia preconditioning can improve self-renewal and migration abilities of USCs by up-regulating autophagy. The purpose of this study was to investigate the specific mechanism by which hypoxia treatment promotes the biological function of USCs. We found that hypoxia treatment upregulated the expression of phosphralated ERK protein without affecting the expression of total ERK protein. Inhibiting ERK signaling with the PD98059 inhibitor decreased cell proliferation, migration and colony formation during hypoxia treatment. Hypoxia increased ATP production, mitochondrial membrane potential and mt-DNA copy number, which were reversed by inhibiting the ERK signal. Additionally, the number of autophagosomes and autophagic lysosomes was significantly lower in PD98059 group than in the hypoxia group. PD98059 treatment inhibited the up-regulation of autophagy related proteins induced by hypoxia. Therefore, this study suggests that hypoxia improves the self-renewal and migration abilities of USCs by upregulating autophagy and mitochondrial function through ERK signaling pathway. This finding may provide a new therapeutic mechanism for hypoxia pretreated USCs as a source of stem cell transplantation."

基金机构：Natural Scientific Research Program of Chongqing Science and Technology Commission [cstc2021jcyj-msxmX0225]; General Project of Basic Research in the Key Laboratory of Child Developmental Diseases of the Ministry of Education [GBRP-202101]

基金资助正文：The present study was supported by the Natural Scientific Research Program of Chongqing Science and Technology Commission (No. cstc2021jcyj-msxmX0225) and the General Project of Basic Research in the Key Laboratory of Child Developmental Diseases of the Ministry of Education (GBRP-202101) .