Probiotics mitigate kidney damage after exposure to Sri Lanka's local groundwater from chronic kidney disease with uncertain etiology (CKDu) prevalent area in zebrafish

作者全名："Bu, Ling-Kang; Jia, Pan-Pan; Li, Wei-Guo; Li, Yong-Zhi; Li, Tian-Yun; Pei, De-Sheng"

作者地址："[Bu, Ling-Kang; Li, Wei-Guo] Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China; [Bu, Ling-Kang; Jia, Pan-Pan; Pei, De-Sheng] Chongqing Med Univ, Sch Publ Hlth, Chongqing 400016, Peoples R China; [Li, Yong-Zhi; Li, Tian-Yun] Chinese Acad Sci, Chongqing Sch Univ, Chongqing Inst Green & Intelligent Technol, CAS, Chongqing 400714, Peoples R China"

通信作者："Pei, DS (通讯作者)，Chongqing Med Univ, Sch Publ Hlth, Chongqing 400016, Peoples R China."

来源：AQUATIC TOXICOLOGY

ESI学科分类：PLANT & ANIMAL SCIENCE

WOS号：WOS:001080003600001

JCR分区：Q1

影响因子：4.1

年份：2023

卷号：262

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Drinking water; CKDu; Probiotics; Zebrafish; Kidney development

摘要："Groundwater in Sri Lanka, contaminated with environmental toxins, is suspected to potentially induce chronic kidney disease of uncertain etiology (CKDu) in humans. This study aims to elucidate the potential mitigating effects of probiotics on kidney damage induced by exposure to this local groundwater (LW) in zebrafish. We used zebrafish as a model organism and exposed them to local groundwater to evaluate the risk of CKDu. Probiotics were then added at a concentration of 108 colony-forming units per milliliter (CFU/mL). Our findings revealed that exposure to local groundwater resulted in abnormalities, such as tail deletion and spinal curvature in zebrafish larvae. However, the addition of probiotics mitigated these effects, improving the hatching rate, heart rate, length, weight, deformity rate, survival rate, and abnormal behavior of zebrafish. It also positively influ-enced the differential expression levels of kidney development and immunity-related genes (dync2h1, foxj1, pkd2, gata3, slc20a1, il1 beta, and lyso). Furthermore, exposure to LW decreased both the diversity and abundance of microbiota in zebrafish larvae. However, treatment with probiotics, such as L. plantarum and L. rhamnosus partially restored the disrupted gut microbiota and significantly impacted the cellular process pathways of the microbial community, as determined by KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis. In conclusion, this study highlights the risks associated with Sri Lanka's local groundwater from a CKDu prevalent area and confirms the beneficial effects of different probiotics. These findings may provide new insights into bacterial function in host kidney health."

基金机构："Chongqing Medical University Talent Project [R4014, R4020]; Chongqing Post-doctoral Innovation Mentor Studio [X7928]; National Natural Science Foundation of China (NSFC) [32200386]; China-Sri Lanka Joint Research and Demonstration Center for Water Technology; China-Sri Lanka Joint Center for Education and Research; Chinese Academy of Sciences, China"

基金资助正文："This work was supported by Chongqing Medical University Talent Project (no. R4014 to D.S.P., and R4020 to P.P.J.) , Chongqing Post-doctoral Innovation Mentor Studio (X7928 D.S.P.) , National Natural Science Foundation of China (NSFC, no.32200386 to P.P.J) , and China-Sri Lanka Joint Research and Demonstration Center for Water Technology, China-Sri Lanka Joint Center for Education and Research, Chinese Academy of Sciences, China."