Alleviation of temporomandibular joint osteoarthritis by targeting RIPK1-mediated inflammatory signalling

作者全名："Cao, Xin; Peng, Sisi; Yan, Ying; Li, Jun; Zhou, Jianping; Dai, Hongwei; Xu, Jie"

作者地址："[Cao, Xin; Peng, Sisi; Yan, Ying; Li, Jun; Zhou, Jianping; Dai, Hongwei; Xu, Jie] Chongqing Med Univ, Coll Stomatol, Chongqing, Peoples R China; [Cao, Xin; Peng, Sisi; Yan, Ying; Li, Jun; Zhou, Jianping; Dai, Hongwei; Xu, Jie] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Cao, Xin; Peng, Sisi; Yan, Ying; Li, Jun; Zhou, Jianping; Dai, Hongwei; Xu, Jie] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China; [Dai, Hongwei; Xu, Jie] Chongqing Med Univ, Coll Stomatol, 426 Songshibei Rd, Chongqing 401147, Peoples R China"

通信作者："Dai, HW; Xu, J (通讯作者)，Chongqing Med Univ, Coll Stomatol, 426 Songshibei Rd, Chongqing 401147, Peoples R China."

来源：JOURNAL OF CELLULAR AND MOLECULAR MEDICINE

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001080728200001

JCR分区：Q2

影响因子：4.3

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Caspase-8; cIAP; MLKL; RIPK1; RIPK3; TNF-alpha

摘要："Temporomandibular joint osteoarthritis (TMJOA), prevalent in adolescents and the elderly, has serious physical and psychological consequences. TMJOA is a degenerative disease of the cartilage and bone, mostly driven by inflammation, and synoviocytes are the first and most important inflammatory factor releasers. Receptor-interacting serine/threonine-protein kinase (RIPK1) promotes inflammatory response and cell death during an array of illnesses. This research aimed to explore the impacts of RIPK1 inhibitor therapy in TMJOA and the mechanism of RIPK1 in inducing inflammation during TMJOA. Herein, inhibition of RIPK1 suppressed the elevated levels of inflammatory factors, nuclear factor kappa B (NF-kappa B), along with markers of apoptosis and necroptosis after tumour necrosis factor (TNF)-alpha/cycloheximide (CHX) treatment in synoviocytes. Moreover, inflammation models were constructed in vivo through complete Freund's adjuvant (CFA) induction and disc perforation, and the findings supported that RIPK1 inhibition protected TMJ articular cartilage against progressive degradation. RIPK1 regulates NF-kappa B activation via cellular inhibitor of apoptosis proteins (cIAP), apoptosis via caspase-8, and necroptosis via RIPK3/mixed lineage kinase domain-like (MLKL) in synoviocytes, which in turn facilitates TMJOA inflammation progression."

基金机构："This study was supported by the National Natural Science Foundation of China (Grant No. 81800999), the Natural Science Foundation of Chongqing, China (Grant Nos. cstc2019jcyj-msxmX0150, cstc2020jcyj-msxmX0191 and CSTB2022NSCQ-MSX0982). [81800999]; National Natural Science Foundation of China [cstc2019jcyj-msxmX0150, cstc2020jcyj-msxmX0191, CSTB2022NSCQ-MSX0982]; Natural Science Foundation of Chongqing, China"

基金资助正文："This study was supported by the National Natural Science Foundation of China (Grant No. 81800999), the Natural Science Foundation of Chongqing, China (Grant Nos. cstc2019jcyj-msxmX0150, cstc2020jcyj-msxmX0191 and CSTB2022NSCQ-MSX0982)."