Silk fibroin hydrogel membranes prepared by a sequential cross-linking strategy for guided bone regeneration

作者全名："Wang, Yuan; Yang, Zhenyu; Chen, Xi; Jiang, Xiaofeng; Fu, Gang"

作者地址："[Wang, Yuan; Yang, Zhenyu; Chen, Xi; Jiang, Xiaofeng; Fu, Gang] Chongqing Med Univ, Stomatol Hosp, Chongqing 401147, Peoples R China; [Wang, Yuan; Yang, Zhenyu; Chen, Xi; Jiang, Xiaofeng; Fu, Gang] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing 401147, Peoples R China; [Wang, Yuan; Yang, Zhenyu; Chen, Xi; Jiang, Xiaofeng; Fu, Gang] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing 401147, Peoples R China"

通信作者："Jiang, XF; Fu, G (通讯作者)，Chongqing Med Univ, Stomatol Hosp, Chongqing 401147, Peoples R China."

来源：JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001081453600001

JCR分区：Q2

影响因子：3.3

年份：2023

卷号：147

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：silk fibroin; Hydrogel; Guided bone regeneration; Photo crosslinking; Ethanol treatment

摘要："Silk fibroin hydrogel is a widely used material for tissue engineering. However, its mechanical properties are the main obstacle to its application to medical bone regeneration barrier membranes. Here, we developed a new hydrogel membrane for guided bone regeneration (GBR). In this study, a sequential crosslinking strategy including photo crosslinking and organic solvent (ethanol) treatment was used to obtain silk fibroin hydrogel membrane (EA-SF). The mechanical properties of EA-SF were significantly enhanced compared to the hydrogel prepared only by photocrosslinking (E-SF). The compressive and tensile strengths of the hydrogel film treated with 75% ethanol for 6 h were 1.18 +/- 0.36 MPa and 0.43 +/- 0.03 MPa, respectively. In vitro cell culture results showed that EA-SF has good biocompatibility and can effectively shield fibroblasts (L929). EA-SF also has excellent in vitro protein hydrolysis stability. In vivo experiments (subcutaneous implantation and calvarial defect experiment) confirmed the stability and barrier functionality of EA-SF. In conclusion, this study demonstrated that ethanol could improve the mechanical properties of silk fibroin hydrogel and extend the scope of their application, making silk fibroin hydrogel a promising GBR material."

基金机构：Joint project of Chongqing Health Commission and Science and Technology Bureau [2023MSXM056]; Chongqing Science and Technology Commis-sion [cstc2019jcyj-msxmX0185]

基金资助正文：This work was supported by the Joint project of Chongqing Health Commission and Science and Technology Bureau (grant number 2023MSXM056) ; and the Chongqing Science and Technology Commis-sion (grant number cstc2019jcyj-msxmX0185) .