A comprehensive review of human trophoblast fusion models: recent developments and challenges

作者全名："Li, Xia; Li, Zhuo-Hang; Wang, Ying-Xiong; Liu, Tai-Hang"

作者地址："[Li, Xia; Liu, Tai-Hang] Chongqing Med Univ, Sch Basic Med Sci, Dept Bioinformat, Chongqing 400016, Peoples R China; [Li, Xia; Li, Zhuo-Hang; Wang, Ying-Xiong; Liu, Tai-Hang] Minist Educ, Joint Int Res Lab Reprod & Dev, Chongqing 400016, Peoples R China; [Li, Zhuo-Hang] Tradit Chinese Med Hosp Yaan, Med Lab Dept, Yaan 625099, Sichuan, Peoples R China"

通信作者："Liu, TH (通讯作者)，Chongqing Med Univ, Sch Basic Med Sci, Dept Bioinformat, Chongqing 400016, Peoples R China.; Wang, YX; Liu, TH (通讯作者)，Minist Educ, Joint Int Res Lab Reprod & Dev, Chongqing 400016, Peoples R China."

来源：CELL DEATH DISCOVERY

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001082011000001

JCR分区：Q1

影响因子：6.1

年份：2023

卷号：9

期号：1

开始页：　

结束页：　

文献类型：Review

关键词：　

摘要："As an essential component of the maternal-fetal interface, the placental syncytiotrophoblast layer contributes to a successful pregnancy by secreting hormones necessary for pregnancy, transporting nutrients, mediating gas exchange, balancing immune tolerance, and resisting pathogen infection. Notably, the deficiency in mononuclear trophoblast cells fusing into multinucleated syncytiotrophoblast has been linked to adverse pregnancy outcomes, such as preeclampsia, fetal growth restriction, preterm birth, and stillbirth. Despite the availability of many models for the study of trophoblast fusion, there exists a notable disparity from the ideal model, limiting the deeper exploration into the placental development. Here, we reviewed the existing models employed for the investigation of human trophoblast fusion from several aspects, including the development history, latest progress, advantages, disadvantages, scope of application, and challenges. The literature searched covers the monolayer cell lines, primary human trophoblast, placental explants, human trophoblast stem cells, human pluripotent stem cells, three-dimensional cell spheres, organoids, and placenta-on-a-chip from 1938 to 2023. These diverse models have significantly enhanced our comprehension of placental development regulation and the underlying mechanisms of placental-related disorders. Through this review, our objective is to provide readers with a thorough understanding of the existing trophoblast fusion models, making it easier to select most suitable models to address specific experimental requirements or scientific inquiries.Establishment and application of the existing human placental trophoblast fusion models."

基金机构："National Natural Science Foundation of China [81801458, 82271707]; Program for Youth Innovation in Future Medicine, Chongqing Medical University [W0068]"

基金资助正文："This work was funded by the National Natural Science Foundation of China (grant numbers 81801458 and 82271707) and Program for Youth Innovation in Future Medicine, Chongqing Medical University (grant numbers W0068)."