Primer Exchange Reaction (PER)-Based Construction of Scaffold for Low-Speed Centrifugation-Based Isolation and Quantitative Analysis of <i>P. aeruginosa and its application in analyzing uterine secretions with intrauterine adhesion</i>

作者全名："Yang, Boping; Wang, Ying; Yan, Xiaohuan; Fen, Qian; Chi, Yugang"

作者地址："[Yang, Boping; Wang, Ying; Yan, Xiaohuan; Chi, Yugang] Chongqing Med Univ, Chongqing Hlth Ctr Women & Children, Dept Gen Gynecol, Women & Childrens Hosp, Chongqing 400037, Peoples R China; [Fen, Qian] Chongqing Med Univ, Hosp Acquired Infect Control Dept, Chongqing Hlth Ctr Women & Children, Women & Childrens Hosp, 120 Longshan St, Chongqing 400037, Peoples R China"

通信作者："Chi, YG (通讯作者)，Chongqing Med Univ, Chongqing Hlth Ctr Women & Children, Dept Gen Gynecol, Women & Childrens Hosp, Chongqing 400037, Peoples R China.; Fen, Q (通讯作者)，Chongqing Med Univ, Hosp Acquired Infect Control Dept, Chongqing Hlth Ctr Women & Children, Women & Childrens Hosp, 120 Longshan St, Chongqing 400037, Peoples R China."

来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001082437000001

JCR分区：Q2

影响因子：3.1

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Pseudomonas aeruginosa (P. aeruginosa); Primer exchange reaction (PER); Low-speed centrifugation; Fluorescent assay

摘要："Efficient isolation and sensitive quantification of Pseudomonas aeruginosa (P. aeruginosa) are crucial for identifying intrauterine infections and preventing the occurrence of intrauterine adhesion (IUA). However, traditional approaches, such as culture-based approach, are time-consuming. Herein, we constructed a detection scaffold by using primer exchange reaction (PER) that integrated the low-speed centrifugation-based isolation and sensitive quantification of target pathogenic bacteria. The established approach possesses several advantages, including (i) the approach is capable of simultaneous isolation and sensitive quantification of target bacteria; (ii) low-speed centrifugation or even manual equipment could be used to isolate target bacteria; and (iii) a low limit of detection was obtained as 54 cfu/mL. Based on this, the approach is a promising approach in analyzing P. aeruginosa from uterine secretions with IUA."

基金机构：The authors thank the financial and technical support from Women and Children's Hospital of Chongqing Medical University.; Women and Children's Hospital of Chongqing Medical University

基金资助正文：The authors thank the financial and technical support from Women and Children's Hospital of Chongqing Medical University.