Cancer-associated fibroblast-related prognostic signature predicts prognosis and immunotherapy response in pancreatic adenocarcinoma based on single-cell and bulk RNA-sequencing

作者全名："Chen, Yajun; Deng, Qican; Chen, Hui; Yang, Jianguo; Chen, Zhenzhou; Li, Juncai; Fu, Zhongxue"

作者地址："[Chen, Yajun; Deng, Qican; Chen, Zhenzhou; Fu, Zhongxue] Chongqing Med Univ, Affiliated Hosp 3, Dept Gen Surg, Chongqing, Peoples R China; [Chen, Hui] Chongqing Gen Hosp, Inst Hepatopancreatobiliary Surg, Chongqing, Peoples R China; [Yang, Jianguo; Fu, Zhongxue] Chongqing Med Univ, Affiliated Hosp 1, Dept Gastrointestinal Surg, Chongqing, Peoples R China; [Li, Juncai] Peoples Hosp Yubei Dist Chongqing, Dept Surg, Chongqing, Peoples R China"

通信作者："Fu, ZX (通讯作者)，Chongqing Med Univ, Affiliated Hosp 3, Dept Gen Surg, Chongqing, Peoples R China.; Fu, ZX (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Gastrointestinal Surg, Chongqing, Peoples R China.; Li, JC (通讯作者)，Peoples Hosp Yubei Dist Chongqing, Dept Surg, Chongqing, Peoples R China."

来源：SCIENTIFIC REPORTS

ESI学科分类：Multidisciplinary

WOS号：WOS:001083957300058

JCR分区：Q1

影响因子：3.8

年份：2023

卷号：13

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Cancer-associated fibroblasts (CAFs) influence many aspects of pancreatic adenocarcinoma (PAAD) carcinogenesis, including tumor cell proliferation, angiogenesis, invasion, and metastasis. A six-gene prognostic signature was constructed for PAAD based on the 189 CAF marker genes identified in single-cell RNA-sequencing data. Multivariate analyses showed that the risk score was independently prognostic for survival in the TCGA (P < 0.001) and ICGC (P = 0.004) cohorts. Tumor infiltration of CD8 T (P = 0.005) cells and naive B cells (P = 0.001) was greater in the low-risk than in the high-risk group, with infiltration of these cells negatively correlated with risk score. Moreover, the TMB score was lower in the low-risk than in the high-risk group (P = 0.0051). Importantly, patients in low-risk group had better immunotherapy responses than in the high-risk group in an independent immunotherapy cohort (IMvigor210) (P = 0.039). The CAV1 and SOD3 were highly expressed in CAFs of PAAD tissues, which revealed by immunohistochemical staining. In summary, this comprehensive analysis resulted in the development of a novel prognostic signature, which was associated with immune cell infiltration, drug sensitivity, and TMB, and could predict the prognosis and immunotherapy response of patients with PAAD."

基金机构：Colorectal Cancer Prevention and Treatment Project of Health Commission of Chongqing [2019ZX003]

基金资助正文：This work was supported by the Colorectal Cancer Prevention and Treatment Project of Health Commission of Chongqing (No. 2019ZX003).