Global and local fat effects on bone mass and quality in obesity INSIGHTS FROM LIPIDOMICS AND 3D IMAGING ASSESSMENT

作者全名："Dai, X.; Liu, B.; Hou, Q.; Dai, Q.; Wang, D.; Xie, B.; Sun, Y.; Wang, B."

作者地址："[Dai, X.; Hou, Q.; Dai, Q.; Wang, D.] Chongqing Med Univ, Chongqing, Peoples R China; [Xie, B.; Wang, B.] Chongqing Med Univ, Dept Gen Practice, Yongchuan Hosp, Chongqing, Peoples R China; [Wang, B.] Chongqing Med Univ, Inst Life Sci, Coll Basic Med, Chongqing, Peoples R China; [Liu, B.] Chongqing Med Univ, Cent Lab, Yongchuan Hosp, Chongqing, Peoples R China; [Sun, Y.] Chongqing Med Univ, Dept Geriatr, Affiliated Hosp 1, Chongqing, Peoples R China; [Dai, Q.] Third Mil Med Univ, Southwest Hosp, Dept Orthoped, Chongqing, Peoples R China; [Wang, D.] Third Mil Med Univ, Southwest Hosp, Dept Stomatol, Chongqing, Peoples R China"

通信作者："Wang, B (通讯作者)，Chongqing Med Univ, Dept Gen Practice, Yongchuan Hosp, Chongqing, Peoples R China.; Wang, B (通讯作者)，Chongqing Med Univ, Inst Life Sci, Coll Basic Med, Chongqing, Peoples R China."

来源：BONE & JOINT RESEARCH

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001085261500007

JCR分区：Q1

影响因子：4.7

年份：2023

卷号：12

期号：9

开始页：580

结束页：589

文献类型：Article

关键词：Fat; Bone mass; Marrow adipose tissue; Lipidomics; 3D imaging; Bone quality

摘要："AimsThe aim of this study was to investigate the global and local impact of fat on bone in obesity by using the diet-induced obese (DIO) mouse model.MethodsIn this study, we generated a diet-induced mouse model of obesity to conduct lipidomic and 3D imaging assessments of bone marrow fat, and evaluated the correlated bone adaptation indices and bone mechanical properties.ResultsOur results indicated that bone mass was reduced and bone mechanical properties were impaired in DIO mice. Lipidomic sequencing and bioinformatic analysis identified 373 differential lipids, 176 of which were upregulated and 197 downregulated. Functional enrichment analysis revealed a significant downregulation of the pathways: fat digestion and absorption (ko04975) and lipolysis regulation in adipocytes (ko04923) in DIO mice, leading to local fat accumulation. The use of 3D imaging confirmed the increase in fat accumulation within the bone marrow cavity of obese mice.ConclusionOur study sheds light on the intricate interplay between fat and bone, and provides a nontoxic and noninvasive method for measuring marrow adipose tissue."

基金机构："National Natural Science Foundation of China [81901424]; Natural Science Foundation of Chongqing, China [CSTB2022NSCQ-MSX0110]; Postgraduate research innovation project of Chongqing, China [CYB22217]; Program for Youth Innovation in Future Medicine, Chongqing Medical University [W0046]"

基金资助正文："center dot The authors disclose receipt of the following financial or material support for the research, authorship, and/or publication of this article: funding from the National Natural Science Foundation of China (81901424) , the Natural Science Foundation of Chongqing, China (CSTB2022NSCQ-MSX0110) , Postgraduate research innovation project of Chongqing, China (CYB22217) , and the Program for Youth Innovation in Future Medicine, Chongqing Medical University (W0046) ."