Perfusion preparation of the rat bladder decellularized scaffold

作者全名："Tan, Jiang; Qiu, Guoping; Wang, Maoqi; Yu, Zhuoyuan; Ling, Xinyi; Aremu, John Ogooluwa; Wang, Chunyu; Liu, Hao; Zhang, Aozhou; Yang, Mei; Gao, Fei"

作者地址："[Tan, Jiang; Yu, Zhuoyuan; Ling, Xinyi; Gao, Fei] Chongqing Med Univ, Affiliated Hosp 1, Dept Urol, Chongqing, Peoples R China; [Tan, Jiang; Qiu, Guoping; Wang, Maoqi; Aremu, John Ogooluwa; Wang, Chunyu; Liu, Hao; Zhang, Aozhou; Yang, Mei] Chongqing Med Univ, Inst Neurosci, Coll Basic Med, Dept Anat, Chongqing, Peoples R China; [Yang, Mei] Chongqing Med Univ, Inst Neurosci, Coll Basic Med, Dept Anat, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China; [Gao, Fei] Chongqing Med Univ, Dept Urol, Affiliated Hosp 1, 1 You Yi RD, Chongqing 400016, Peoples R China"

通信作者："Yang, M (通讯作者)，Chongqing Med Univ, Inst Neurosci, Coll Basic Med, Dept Anat, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China.; Gao, F (通讯作者)，Chongqing Med Univ, Dept Urol, Affiliated Hosp 1, 1 You Yi RD, Chongqing 400016, Peoples R China."

来源：REGENERATIVE THERAPY

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001086137300001

JCR分区：Q2

影响因子：3.4

年份：2023

卷号：24

期号：　

开始页：499

结束页：506

文献类型：Article

关键词：Urinary bladder; Bladder acellular matrix; Bladder regeneration; Tissue engineering

摘要："Introduction: Bladder reconstruction is a huge challenge in the field of urology. In recent years, perfusion methods have brought promising results in the field of tissue engineering. We prepared bladder decellularized scaffolds by improved perfusion, which may be suitable for bladder reconstruction.Methods: We prepared decellularized scaffolds of rat bladder by perfusion of SDS (0.5% sodium dodecyl sulfate), SDS-SDC (0.5% sodium dodecyl sulfate thorn 0.5% sodium deoxycholate). Histological characteristics of bladder decellularized scaffolds were assessed by Hematoxylin and eosin, Masson, and DAPI staining. Moreover, we also prepared a murine bladder transplantation model to evaluate the regenerative potential of scaffolds.Results: Hematoxylin and eosin, Masson, and DAPI staining indicated almost no cellular component residues in the SDS-SDC group. Histological analysis (hematoxylin and eosin staining, Masson staining), CD31 and F4/80 staining analysis, one month after implantation, revealed that the decellularized scaffolds had regenerative characteristics, and the SDS-SDC scaffold had better regenerative properties than the SDS scaffold.Conclusions: We successfully prepared the decellularized scaffold for the rat bladder by perfusion. Our results showed that the SDS-SDC scaffold had better decellularization efficiency and reconstruction ability than the SDS scaffold, which provides a new perspective on bladder reconstruction materials."

基金机构："National Natural Science Foundation of China [82370691, 81971230, 81671312]; Chongqing Science and health joint project [2020GDRC007]; Senior Medical Talents Program of Chongqing for Yong and Middle-aged [204216qn]; Reserve Talents Program for academic Leaders of the First Affiliated Hospital of Chongqing Medical University [XKTS070]; Natural Science Foundation of Chongqing, China [CSTB2023NSCQ-MSX0072, CSTB2023NSCQ-MSX0565, CSTB2023NSCQ-BHX0192]"

基金资助正文："This work was supported by the National Natural Science Foundation of China to Fei Gao [NO. 82370691] and Mei Yang [NO. 81971230, 81671312] . Furthermore, it is supported by Chongqing Science and health joint project [NO. 2020GDRC007] , and supported by Senior Medical Talents Program of Chongqing for Yong and Middle-aged [NO. 204216qn] and Reserve Talents Program for academic Leaders of the First Affiliated Hospital of Chongqing Medical University [NO. XKTS070] to Fei Gao. Finally, this work was also sponsored by Natural Science Foundation of Chongqing, China to Fei Gao (No. CSTB2023NSCQ-MSX0072) , to Mei Yang (No. CSTB2023NSCQ-MSX0565) and to John Ogooluwa Aremu (No. CSTB2023NSCQ-BHX0192."