Let-7a promotes periodontal bone regeneration of bone marrow mesenchymal stem cell aggregates via the Fas/FasL-autophagy pathway

作者全名："Yang, Shiyao; Gao, Jing; Chen, Meng; Sun, Yuting; Qiao, Xin; Mao, Hongchen; Guo, Li; Yu, Yang; Yang, Deqin"

作者地址："[Yang, Shiyao; Gao, Jing; Sun, Yuting; Qiao, Xin; Mao, Hongchen; Guo, Li; Yu, Yang; Yang, Deqin] Chongqing Med Univ, Dept Endodont, Stomatol Hosp, Chongqing 404100, Peoples R China; [Yang, Shiyao; Gao, Jing; Sun, Yuting; Mao, Hongchen; Guo, Li; Yu, Yang; Yang, Deqin] Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Stomatol Hosp, Chongqing, Peoples R China; [Yang, Shiyao; Gao, Jing; Sun, Yuting; Qiao, Xin; Mao, Hongchen; Guo, Li; Yu, Yang; Yang, Deqin] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China; [Chen, Meng] Army Med Univ, Daping Hosp, Dept Oral & Maxillofacial Surg, Chongqing, Peoples R China; [Qiao, Xin] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China"

通信作者："Yu, Y; Yang, DQ (通讯作者)，Chongqing Med Univ, Dept Endodont, Stomatol Hosp, Chongqing 404100, Peoples R China."

来源：JOURNAL OF CELLULAR AND MOLECULAR MEDICINE

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001086229400001

JCR分区：Q2

影响因子：4.3

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：BMMSCs; cell-aggregates; Fas/FasL; microRNA; periodontal regeneration

摘要："Periodontal bone regeneration using bone marrow mesenchymal stem cell (BMMSC) transplantation is a promising method; however, the method for osteogenic differentiation of BMMSCs needs to be improved. In this research, we sought to identify the roles of let-7a in the osteogenesis of BMMSCs and to provide a potential method for periodontal bone regeneration. Our previous study revealed that Fas/FasL is a target of let-7a. In this study, we demonstrated that let-7a overexpression significantly enhanced BMMSC-CAs osteogenesis both in vitro and in vivo. Mechanistically, upregulation of Fas/FasL using the rfas/rfaslg plasmid obstructed the osteogenesis of BMMSCs by inhibiting autophagy. Furthermore, we confirmed that overexpression of let-7a activated autophagy and alleviated the inhibited osteogenesis by the autophagy inhibitor 3-MA and the rfas/rfaslg plasmid of BMMSCs. In general, our findings showed that let-7a promoted the osteogenesis of BMMSCs through the Fas/FasL-autophagy pathway, suggesting that the application of let-7a in BMMSC-CAs based periodontal bone regeneration could be a promising strategy."

基金机构："The National Natural Science Foundation of China (No. 31970783 to DY, No. 32270888 to DY, No. 81800979 to YY), Chongqing Medical Universityapos;s Program for Top Talent Distinguished Professor (No. [2021] 215), and Chongqing Medical Universityapos;s Prog [31970783, 32270888, 81800979]; National Natural Science Foundation of China [[2021] 215]; Chongqing Medical Universityapos;s Program for Top Talent Distinguished Professor [W0060]; Chongqing Medical Universityapos;s Program for Youth Innovation in Future Medicine"

基金资助正文："The National Natural Science Foundation of China (No. 31970783 to DY, No. 32270888 to DY, No. 81800979 to YY), Chongqing Medical University & apos;s Program for Top Talent Distinguished Professor (No. [2021] 215), and Chongqing Medical University & apos;s Program for Youth Innovation in Future Medicine (No. W0060 to DY) provided funding for this study."