Overexpression of SH2D1A promotes cancer progression and is associated with immune cell infiltration in hepatocellular carcinoma via bioinformatics and in vitro study

作者全名："Xiang, Qian-Ming; Jiang, Ni; Liu, Yue-Feng; Wang, Yuan-Biao; Mu, De-An; Liu, Rong; Sun, Lu-Yun; Zhang, Wei; Guo, Qiang; Li, Kai"

作者地址："[Xiang, Qian-Ming; Guo, Qiang] Hubei Univ Med, Taihe Hosp, Dept Cardiothorac Surg, Shiyan, Peoples R China; [Xiang, Qian-Ming; Liu, Rong; Sun, Lu-Yun] Kunming Med Univ, Affiliated Hosp 1, Dept Gen Surg, Kunming, Peoples R China; [Jiang, Ni] Chongqing Med Univ, Dept Obstet & Gynecol, Women & Childrens Hosp, Chongqing, Peoples R China; [Liu, Yue-Feng] Hubei Univ Med, Taihe Hosp, Dept Ophthalmol Surg, Shiyan, Peoples R China; [Wang, Yuan-Biao] Kunming Med Univ, Dept Yunnan Tumor Res Inst, Affiliated Hosp 3, Kunming, Peoples R China; [Mu, De-An; Zhang, Wei; Li, Kai] Peoples Hosp Jianyang City, Dept Hepatobiliary & Pancreat Surg, Jianyang, Peoples R China"

通信作者："Guo, Q (通讯作者)，Hubei Univ Med, Taihe Hosp, Dept Cardiothorac Surg, Shiyan, Peoples R China.; Zhang, W; Li, K (通讯作者)，Peoples Hosp Jianyang City, Dept Hepatobiliary & Pancreat Surg, Jianyang, Peoples R China."

来源：BMC CANCER

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001087048900003

JCR分区：Q2

影响因子：3.4

年份：2023

卷号：23

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：SH2 domain containing 1A; Hepatocellular carcinoma; Immune microenvironment; Prognosis; The Cancer Genome Atlas

摘要："BackgroundSH2 domain containing 1A (SH2D1A) expression has been linked to cancer progression. However, the functions of SH2D1A in hepatocellular carcinoma (HCC) have not been reported.MethodsThe effects of SH2D1A on the proliferation, migration, and invasion of HCC cells and the related pathways were re-explored in cell models with SH2D1A overexpression using the CCK-8, migration and invasion assays and western blotting. The functions and mechanisms of genes co-expressed with SH2D1A were analyzed using gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The relationship between SH2D1A expression and immune microenvironment features in HCC was explored.ResultsElevated SH2D1A expression promoted cell proliferation, migration, and invasion, which was related to the overexpression of p-Nf-kappa B and BCL2A1 protein levels in HCC. SH2D1A expression was related to the immune, stromal, and ESTIMATE scores, and the abundance of immune cells, such as B cells, CD8+ T cells, and T cells. SH2D1A expression was significantly related to the expression of immune cell markers, such as PDCD1, CD8A, and CTLA4 in HCC.ConclusionSH2D1A overexpression was found to promote cell growth and metastasis via the Nf-kappa B signaling pathway and may be related to the immune microenvironment in HCC. The findings indicate that SH2D1A can function as a biomarker in HCC."

基金机构：Not applicable.

基金资助正文：Not applicable.