Portable dual-channel blood enzyme analyzer for point-of-care liver function detection

作者全名："Sang, Jingwei; Cheng, Jie; Hu, Honghua; Liu, Ke; Guo, Jiuchuan; Guo, Jinhong"

作者地址："[Sang, Jingwei; Liu, Ke; Guo, Jiuchuan] Univ Elect Sci & Technol China, Chengdu 611731, Peoples R China; [Cheng, Jie; Guo, Jinhong] Shanghai Jiao Tong Univ, Sch Sensing Sci & Engn, Shanghai 200240, Peoples R China; [Hu, Honghua] Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Dept Lab Med, Chengdu, Peoples R China; [Hu, Honghua] Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Sichuan Prov Key Lab Human Dis Gene Study, Chengdu, Peoples R China; [Guo, Jinhong] Chongqing Med Univ, Coll Lab Med, MOE Key Lab Lab Med Diagnost, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China"

通信作者："Guo, JH (通讯作者)，Shanghai Jiao Tong Univ, Sch Sensing Sci & Engn, Shanghai 200240, Peoples R China.; Guo, JH (通讯作者)，Chongqing Med Univ, Coll Lab Med, MOE Key Lab Lab Med Diagnost, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China."

来源：ANALYST

ESI学科分类：CHEMISTRY

WOS号：WOS:001088062500001

JCR分区：Q2

影响因子：3.6

年份：2023

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文献类型：Article; Early Access

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摘要："Because the liver is an important metabolic center in the human body, the reliability and timeliness of chronic liver disease diagnosis are particularly important. Alanine aminotransferase and aspartate transaminase are the two most important liver function indicators, and their test results are crucial in the diagnosis of liver diseases. However, the simultaneous detection of these two indicators is currently restricted by the need for expensive equipment and complicated detection processes. This study proposes a portable dual-channel blood enzyme analyzer (BEA) for point-of-care-testing. The device uses photometric reflectance to quantify the enzyme concentration by evaluating the reflected light intensity. The BEA also precisely controls and maintains the temperature at 37 degrees C +/- 0.1 degrees C in the dual-channel assay. We assessed the responses of this system within a clinically relevant range by testing blood samples from a local hospital. The test verified that BEA for ALT and AST achieved a detection limit of 3.5 U L-1 and 4 U L-1, detection range of 4-350 U L-1 and 4-250 U L-1, coefficients of variation (CV) that were both less than 10%, and a linear correlation coefficient of 0.9827 and 0.9714 compared with a high-precision clinical biochemistry analyzer (Roche Cobas C702), respectively. We realized remote data analysis and storage through connection with smartphones, which can be applied to remote diagnostics and preventative personal disease management. Therefore, BEA has broad application prospects in the future internet of medical things. Because the liver is an important metabolic center in the human body, the reliability and timeliness of chronic liver disease diagnosis are particularly important."

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