<i>mitfa</i> deficiency promotes immune vigor and potentiates antitumor effects in zebrafish

作者全名："Bian, Wan-Ping; Xie, Shao-Lin; Wang, Chao; Martinovich, Grigoriy G.; Ma, Yan-Bo; Jia, Pan-Pan; Pei, De-Sheng"

作者地址："[Bian, Wan-Ping; Ma, Yan-Bo] Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China; [Xie, Shao-Lin; Wang, Chao; Jia, Pan-Pan; Pei, De-Sheng] Chongqing Med Univ, Sch Publ Hlth, Chongqing 400016, Peoples R China; [Martinovich, Grigoriy G.] Belarusian State Univ, Minsk 220030, BELARUS"

通信作者："Pei, DS (通讯作者)，Chongqing Med Univ, Sch Publ Hlth, Chongqing 400016, Peoples R China."

来源：FISH & SHELLFISH IMMUNOLOGY

ESI学科分类：PLANT & ANIMAL SCIENCE

WOS号：WOS:001088547100001

JCR分区：Q1

影响因子：4.1

年份：2023

卷号：142

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：CRISPR/Cas9; mitfa-/-Immune response; Zebrafish

摘要："The mitfa gene is a well-known transcription factor associated with microphthalmia and is essential for early melanophore development. However, little is known about how mitfa affects the immune system. Here, we generated a novel mitfa knock-out zebrafish line using the CRISPR/Cas9 system. The mitfa-/- zebrafish exhibited reduced melanin levels compared to the nacre mutant. We investigated the impact on the immune system after exposure to Edwardsiella tarda and bifenazate in zebrafish larvae, and observed that the macrophage numbers were reduced in both treated groups. Remarkably, the expression levels of immune-related genes exhibited significant increases after bacterial challenge or bifenazate exposure in the mitfa- /- zebrafish, except for tlr4 and rela. Furthermore, we conducted xenograft experiments using mouse B16 melanoma cells. Notably, the cancer cells didn't show a high cell migration ratio, implying that the immune system was highly activated after the loss of mifta. Taken together, our findings suggest that mitfa-/- zebrafish serve as a valuable model for investigating the relationship between the immune system and melanocytes, providing new insights into the role of mitfa in immune responses."

基金机构："Chongqing Medical University Talent Project [R4014, R4020]; Chongqing Postdoctoral Innovation Mentor Studio [X7928]; Ecological Environment Investigation and Ecological Restoration Technology Demonstration Project [5000002021BF40001]; National Natural Science Foundation of China [32200386]; China-Sri Lanka Joint Research and Demonstration Center for Water Technology; China-Sri Lanka Joint Center for Education and Research, Chinese Academy of Sciences, China"

基金资助正文："This work was supported by Chongqing Medical University Talent Project (No. R4014 and R4020) , Chongqing Postdoctoral Innovation Mentor Studio (X7928 D.S.P.) , Ecological Environment Investigation and Ecological Restoration Technology Demonstration Project in the Water-level-fluctuating Area (Chongqing Section) of the Three Gorges Reservoir (No. 5000002021BF40001) ,National Natural Science Foundation of China (No.32200386) , and China-Sri Lanka Joint Research and Demonstration Center for Water Technology, China-Sri Lanka Joint Center for Education and Research, Chinese Academy of Sciences, China."