"Bacterial growth stage determines the yields, protein composition, and periodontal pathogenicity of Porphyromonas gingivalis outer membrane vesicles"

作者全名："Mao, Hongchen; Gong, Ting; Sun, Yuting; Yang, Shiyao; Qiao, Xin; Yang, Deqin"

作者地址："[Mao, Hongchen; Gong, Ting; Sun, Yuting; Yang, Shiyao; Qiao, Xin; Yang, Deqin] Chongqing Med Univ, Dept Endodont, Stomatol Hosp, Chongqing, Peoples R China; [Mao, Hongchen; Gong, Ting; Sun, Yuting; Qiao, Xin; Yang, Deqin] Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Stomatol Hosp, Chongqing, Peoples R China; [Mao, Hongchen; Gong, Ting; Sun, Yuting; Yang, Shiyao; Qiao, Xin; Yang, Deqin] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China; [Yang, Shiyao] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China"

通信作者："Yang, DQ (通讯作者)，Chongqing Med Univ, Dept Endodont, Stomatol Hosp, Chongqing, Peoples R China.; Yang, DQ (通讯作者)，Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Stomatol Hosp, Chongqing, Peoples R China.; Yang, DQ (通讯作者)，Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China."

来源：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:001089154300001

JCR分区：Q1

影响因子：4.6

年份：2023

卷号：13

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Porphyromonas gingivalis; outer membrane vesicles; periodontal pathogenicity; rat periodontitis model; caspase-1/NLRP3/IL-1 beta

摘要："Introduction P. gingivalis (W83), as the keystone pathogen in chronic periodontitis, has been found to be tightly bound to systemic diseases. Outer membrane vesicles (OMVs) produced by P. gingivalis (W83) are thought to serve key functions in bacterial virulence and pathogenicity. This study aims to comprehend the biological functions of P. gingivalis OMVs isolated from different growth stages by comparing their physicochemical properties and pathogenicity.Methods Protein composition was analyzed via isotope-labeled relative and absolute quantification (iTRAQ). Macrophage polarization and the expression of IL-6 and IL-1 beta were detected. The proliferation, migration, osteogenic differentiation, and IL-1b/NLRP3 expression of periodontal ligament stem cells (PDLSCs) were evaluated. P. gingivalis/P. gingivalis OMVs-induced periodontal models were also constructed in Sprague Dawley rats.Results The protein composition of P. gingivalis OMVs isolated from different growth stages demonstrated obvious differences ranging from 25 KDa to 75 KDa. In the results of flow cytometry, we found that in vitro experiments the M1 subtype of macrophages was more abundant in the late-log OMVs and stationary OMVs groups which boosted the production of inflammatory cytokines more than pre-log OMVs. Compared to pre-log OMVs, late-log OMVs and stationary OMVs had more pronounced inhibitory effects on proliferation, migration, and early osteogenesis of PDLSCs. The NLRP3 inflammasome was activated to a larger extent in the stationary OMVs group. Micro-computed tomography (Micro CT), hematoxylin-eosin staining (HE), and tartrate acid phosphatase (TRAP) results showed that the periodontal damage in the stationary OMVs group was worse than that in the pre-log OMVs and late-log OMVs group, but almost equal to that in the positive control group (P. gingivalis).Discussion In general, both in vivo and in vitro experiments showed that late-log OMVs and stationary OMVs have more significant pathogenicity in periodontal disease."

基金机构："The authors declare financial support was received for the research, authorship, and/or publication of this article. This study was supported by the grants from the Project Supported by the National Natural Science Foundation of China (No.31970783 to DY, N [31970783, 32270888]; National Natural Science Foundation of China [2021215]; Program for Top talent Distinguished Professor from Chongqing Medical University [W0060]; Program for Youth Innovation in Future Medicine from Chongqing Medical University [cstc2021jcyj bshX0176]; Natural Science Foundation of Chongqing, China"

基金资助正文："We thank the members of the Stomatological Hospital of Chongqing Medical University for their helpful discussions.r The authors declare financial support was received for the research, authorship, and/or publication of this article. This study was supported by the grants from the Project Supported by the National Natural Science Foundation of China (No.31970783 to DY, NO.32270888 to DY), Program for Top talent Distinguished Professor from Chongqing Medical University to DY(NO. 2021215), Program for Youth Innovation in Future Medicine from Chongqing Medical University (No. W0060 to DY), Natural Science Foundation of Chongqing, China (No. cstc2021jcyj bshX0176 to TG)."