<i>H19</i> PROMOTER DNA METHYLATION IS LOWER AMONG EARLY ABORTION PATIENTS UNDERGOING IVF EMBRYO TRANSFER

作者全名："Han, Lei; Zhang, Xianghui; Wu, Xiaohan; Xu, Huishu; Zhang, Baolin; Pang, Yiwei; Ding, Peihui; Zhang, Caiying; Wang, Yanlin; An, Jiajia"

作者地址："[Han, Lei; Zhang, Xianghui; Wu, Xiaohan; Xu, Huishu; Zhang, Baolin; Pang, Yiwei; Ding, Peihui; Wang, Yanlin] Binzhou Med Univ Hosp, Dept Reprod Med, Binzhou 256603, Shandong, Peoples R China; [Han, Lei] Chongqing Med Univ, Affiliated Hosp 3, Dept Obstet & Gynecol, Chongqing 401120, Peoples R China; [Han, Lei] Zunyi Med Univ, Dept Reprod Med, Maternal & Child Hlth Hosp, Zunyi 563000, Guizhou, Peoples R China; [Zhang, Baolin] Binzhou Cent Hosp, Dept Obstet & Gynecol, Binzhou 251700, Shandong, Peoples R China; [Zhang, Caiying] Binzhou Med Univ Hosp, Dept Postgrad Student Off, Binzhou 256603, Shandong, Peoples R China; [An, Jiajia] Binzhou Med Univ Hosp, Dept Clin Lab, Binzhou 256603, Shandong, Peoples R China"

通信作者："Wang, YL (通讯作者)，Binzhou Med Univ Hosp, Dept Reprod Med, Binzhou 256603, Shandong, Peoples R China.; An, JJ (通讯作者)，Binzhou Med Univ Hosp, Dept Clin Lab, Binzhou 256603, Shandong, Peoples R China."

来源：CLINICAL AND INVESTIGATIVE MEDICINE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001089438500001

JCR分区：Q4

影响因子：1.2

年份：2023

卷号：46

期号：3

开始页：E13

结束页：E18

文献类型：Article

关键词：　

摘要："Background: H19 is the first long noncoding RNA (lncRNA) found to be associated with gene imprinting. It is highly expressed in the embryonic stage and may have important regulatory effects on human embryonic development. We investigated the differences between the levels of H19 promoter DNA methylation in the chorionic villi of patients who experienced spontaneous abortion (SA) following in vitro fertilization embryo transfer (IVF-ET) and those of patients with a normal early pregnancy (NEP). We also analyzed the associated DNA methyltransferase (DNMT) activity. Methods: Chorionic villus tissue from patients with SA and NEP were collected. The DNA methylation levels of two CpG islands in the promoter region of the H19 gene in the two groups were detected by bisulfite sequencing, and the mRNA expression of DNMTs was analyzed by real-time polymerase chain reaction. Results: The sample size of each group was 32, and there were no significant differences in baseline data, including age, parity, and body mass index, between the two groups. Among the 7 CpG islands measured, the methylation rates of 3 CpG islands (CpG 1, 6, and 7) were significantly lower in the SA group than in the NEP group (P < 0.01). The methylation levels of the other 4 CpG islands were not significantly different between the two groups. There were no differences in the expression of DNMT1 between the two groups (P > 0.05), but DNMT3a and DNMT3b RNA levels were significantly lower in SA group than in the NEP group (P < 0.01). Conclusions: The lower H19 promoter DNA methylation levels found in the chorionic villi of patients with SA patients following IVF-ET may be explained by decreased expression of DNMT3a and DNMT3b."

基金机构：Scientific Research Fund of Binzhou Medical University [BY2021KYQD34]; Scientific Research Initiation Fund of Affiliated Hospital of Binzhou Medical University Hospital [2021-03]; Science and Technology Fund Project of Guizhou Provincial Health Commission [gzwkj2021-302]; Science and Technology Plan Project of Honghuagang District in Zunyi City [07]; Science and Technology Plan Project of Zunyi City; [271]

基金资助正文：This work was supported by the Scientific Research Fund of Binzhou Medical University (BY2021KYQD34) ; Scientific Research Initiation Fund of Affiliated Hospital of Binzhou Medical University Hospital (2021-03) . Science and Technology Fund Project of Guizhou Provincial Health Commission (gzwkj2021-302) ; Science and Technology Plan Project of Honghuagang District in Zunyi City (Zun Hong Ke He She [2020] No. 07) ; Science and Technology Plan Project of Zunyi City (Zun Yi Ke He HZ [2020] No. 271) .