Reprogramming macrophages via immune cell mobilized hydrogel microspheres for osteoarthritis treatments

作者全名："Xiao, Pengcheng; Han, Xiaoyu; Huang, Yanran; Yang, Jianye; Chen, Li; Cai, Zhengwei; Hu, Ning; Cui, Wenguo; Huang, Wei"

作者地址："[Xiao, Pengcheng; Han, Xiaoyu; Huang, Yanran; Yang, Jianye; Chen, Li; Hu, Ning; Huang, Wei] Chongqing Med Univ, Affiliated Hosp 1, Dept Orthoped, Orthopaed Res Lab, Chongqing 400016, Peoples R China; [Han, Xiaoyu; Cai, Zhengwei; Cui, Wenguo] Shanghai Jiao Tong Univ, Ruijin Hosp,Sch Med,Dept Orthopaed, Shanghai Inst Traumatol & Orthopaed, Shanghai Key Lab Prevent & Treatment Bone & Joint, 197 Ruyin 2nd Rd, Shanghai 200025, Peoples R China"

通信作者："Hu, N; Huang, W (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Orthoped, Orthopaed Res Lab, Chongqing 400016, Peoples R China.; Cui, WG (通讯作者)，Shanghai Jiao Tong Univ, Ruijin Hosp,Sch Med,Dept Orthopaed, Shanghai Inst Traumatol & Orthopaed, Shanghai Key Lab Prevent & Treatment Bone & Joint, 197 Ruyin 2nd Rd, Shanghai 200025, Peoples R China."

来源：BIOACTIVE MATERIALS

ESI学科分类：　

WOS号：WOS:001090190200001

JCR分区：Q1

影响因子：18.9

年份：2024

卷号：32

期号：　

开始页：242

结束页：259

文献类型：Article

关键词：Engineered cell membrane vesicles; Hydrogel microspheres; Macrophage reprogram; Osteoarthritis

摘要："Regulating macrophage activation precisely is crucial in treating chronic inflammation in osteoarthritis (OA). However, the stable pro-inflammatory state and deep distribution of macrophages in vivo pose a great challenge to treatment. In this study, inspired by the innate immune, immune cell mobilized hydrogel microspheres were constructed by microfluidic methods and load chemokines, macrophage antibodies and engineered cell mem-brane vesicles (sEVs) via covalent and non-covalent junctions. The immune cell mobilized hydrogel micro-spheres, based on a mixture of streptavidin grafted hyaluronic acid methacrylate (HAMA-SA) and Chondroitin sulfate methacrylate (ChSMA) microspheres (HCM), can recruit, capture and reprogram proinflammatory macrophages in the joint cavity to improve the joint inflammatory microenvironment. In vitro experiments demonstrated that immune cell mobilized hydrogel microspheres had excellent macrophage recruitment, capture, and reprogramming abilities. Pro-inflammatory macrophages can be transformed into anti-inflammatory macrophages with an efficiency of 88.5 %. Animal experiments also revealed significant reduction in synovial inflammation and cartilage matrix degradation of OA. Therefore, the immune cell mobilized hydrogel micro-spheres may be an effective treatment of OA inflammation for the future."

基金机构："National Natural Science Foundation of China-Joint Fund Project [U22A20284]; National Natural Science Foundation of China [81972069, 82202724]; Doctoral Cultivating Project of the First Affiliated Hospital of Chongqing Medical University [CYYY-BSYJSCXXM-202227, 202204]"

基金资助正文："This work was supported by the National Natural Science Foundation of China-Joint Fund Project (U22A20284) ; the National Natural Science Foundation of China (81972069, 82202724) ; Doctoral Cultivating Project of the First Affiliated Hospital of Chongqing Medical University (CYYY-BSYJSCXXM-202227; 202204) ."