Mechanical loading and autophagy: A study on the BoNT-A injection-induced condylar cartilage degeneration

作者全名："Hou, Siyu; Peng, Sisi; Dai, Hongwei; Song, Jinlin; Xu, Ling; Zhou, Jianping; Li, Lingjie"

作者地址："[Hou, Siyu; Peng, Sisi; Dai, Hongwei; Song, Jinlin; Xu, Ling; Zhou, Jianping; Li, Lingjie] Chongqing Med Univ, Coll Stomatol, Chongqing 401147, Peoples R China; [Hou, Siyu; Peng, Sisi; Dai, Hongwei; Song, Jinlin; Zhou, Jianping; Li, Lingjie] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing 401147, Peoples R China; [Hou, Siyu; Peng, Sisi; Dai, Hongwei; Song, Jinlin; Xu, Ling; Zhou, Jianping; Li, Lingjie] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing 401147, Peoples R China"

通信作者："Zhou, JP; Li, LJ (通讯作者)，Chongqing Med Univ, Coll Stomatol, Chongqing 401147, Peoples R China."

来源：ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001092192100001

JCR分区：Q1

影响因子：3.8

年份：2023

卷号：749

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Botulinum toxin type A; Temporomandibular joint

摘要："Botulinum toxin A (BoNT-A) has emerged as a treatment option for temporomandibular disorder (TMD). By injecting BoNT-A into the masseter muscle, it is possible to reduce mechanical loading on the temporomandibular joint (TMJ). However, numerous prior studies have indicated excessive reduction in mechanical loading can have detrimental effects on TMJ cartilage. This study proposes that autophagy, a process influenced by mechanical loading, could play a role in BoNT-A-induced mandibular condyle cartilage degeneration. To explore this hypothesis, we employed both BoNT-A injection and an excessive biting model to induce variations in mechanical loading on the condyle cartilage of C57BL/6 mice, thereby simulating an increase and decrease in mechanical loading, respectively. Results showed a significant reduction in cartilage thickness and downregulation of Runt-related transcription factor 2 (Runx2) expression in chondrocytes following BoNT-A injection. In vitro experiments demonstrated that the reduction of Runx2 expression in chondrocytes is associated with autophagy, possibly dependent on decreased YAP expression induced by low mechanical loading. This study reveals the potential involvement of the YAP/LC3/Runx2 signaling pathway in BoNT-A mediated mandibular condylar cartilage degeneration."

基金机构："National Natural Science Founda-tion of China [82001081]; Natural Science Foundation of Chongq-ing, China [csts2020jcyj-bshX0022, cstc2020jcyj-msxmX0191, CSTB2022NSCQ-MSX0982]; Postdoctoral Science Foundation of China [2020M683266]; Postdoctoral Program for Innovative Talents of Chongqing, China [2010010006080066]; Open Project of State Key Laboratory of Ultrasonic Medical Engineering [2022KFKT003]"

基金资助正文："<B>Funding</B> This study was supported by the National Natural Science Founda-tion of China (No. 82001081) ; Natural Science Foundation of Chongq-ing, China (No. csts2020jcyj-bshX0022, No. cstc2020jcyj-msxmX0191, and No. CSTB2022NSCQ-MSX0982) ; Postdoctoral Science Foundation of China (No. 2020M683266) ; Postdoctoral Program for Innovative Talents of Chongqing, China (No. 2010010006080066) ; Open Project of State Key Laboratory of Ultrasonic Medical Engineering (No. 2022KFKT003) ."