Hydrogen sulfide inhibits the rupture of fetal membranes throngh anti-aging pathways

作者全名："Wang, Jie; Xu, Jiacheng; Chao, Bingdi; Liu, Hongli; Xie, Lumei; Qi, Hongbo; Luo, Xin"

作者地址："[Wang, Jie; Xu, Jiacheng; Chao, Bingdi; Liu, Hongli; Xie, Lumei; Luo, Xin] Chongqing Med Univ, Affiliated Hosp 1, Dept Obstet, Chongqing 400016, Peoples R China; [Wang, Jie; Xu, Jiacheng; Chao, Bingdi; Liu, Hongli; Xie, Lumei; Qi, Hongbo; Luo, Xin] Chongqing Med Univ, Chongqing Key Lab Maternal & Fetal Med, Chongqing 400016, Peoples R China; [Wang, Jie; Qi, Hongbo] Chongqing Med Univ, Women & Childrens Hosp, Chongqing 401147, Peoples R China; [Qi, Hongbo] Chongqing Med Univ CQMU WCH, Women & Childrens Hosp, Dept Obstet & Gynecol, 120 Longshan Rd, Chongqing 401147, Peoples R China; [Luo, Xin] Chongqing Med Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, 1 Youyi Rd, Chongqing 400016, Peoples R China"

通信作者："Qi, HB (通讯作者)，Chongqing Med Univ CQMU WCH, Women & Childrens Hosp, Dept Obstet & Gynecol, 120 Longshan Rd, Chongqing 401147, Peoples R China.; Luo, X (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, 1 Youyi Rd, Chongqing 400016, Peoples R China."

来源：PLACENTA

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001093306800001

JCR分区：Q1

影响因子：3

年份：2023

卷号：143

期号：　

开始页：22

结束页：33

文献类型：Article

关键词：Fetal membranes; Preterm premature rupture of membranes (pPROM); Hydrogen sulfide (H2S); Matrix metalloproteinases (MMPs); Epithelial-mesenchymal translation (EMT)

摘要："Introduction: To investigate the relationship between hydrogen sulfide(H2S) and the senescence level of the fetal membranes, and to elucidate how H2S affects the integrity of the fetal membranes.Methods: The H2S and the senescence levels of fetal membranes, and the expressions of H2S synthase CBS and CSE were detected in the preterm (PT) group and the preterm premature ruptured membranes (pPROM) group. The effects of H2S donors and knockdown of CBS on the senescence level of amniotic epithelial cells, and the expression level of matrix metalloproteinases (MMPs) and epithelial-mesenchymal translation (EMT) were observed.Results: The level of H2S in the fetal membranes in the pPROM group is significantly lower than that in the PT group matched for gestational age. The level of H2S is negatively correlated with the senescence level of fetal membranes. Treatment with H2S donors reduced cell senescence and MMPs expression, but did not affect EMT. CBS siRNA transfection accelerated the senescence of amniotic epithelial cells, and promoted the expression of MMPs and EMT occurrence, but L-cysteine could reverse these effects.Discussion: Our study suggests that H2S, through its anti-aging effect, can influence the expression of MMPs and EMT, thereby contributing to the maintenance of fetal membrane integrity."

基金机构："National Natural Science Foundation of China [82171668, 82171662, U21A20346]; ""111 program"" of the Ministry of Edu-cation P.R.C; State Administration of Foreign Experts Affairs P.R. C."

基金资助正文："This study was funded by National Natural Science Foundation of China (82171668, 82171662, and U21A20346) . We would like to appreciate the support from the ""111 program"" of the Ministry of Edu-cation P.R.C and the State Administration of Foreign Experts Affairs P.R. C."