Emodin improves glucose metabolism and ovarian function in PCOS mice via the HMGB1/TLR4/NF-κB molecular pathway

作者全名："Otoo, Antonia; Czika, Armin; Lamptey, Jones; Yang, Jun-Pu; Feng, Qian; Wang, Mei-Jiao; Wang, Ying-Xiong; Ding, Yu-Bin"

作者地址："[Otoo, Antonia; Wang, Ying-Xiong; Ding, Yu-Bin] Chongqing Med Univ, Women & Childrens Hosp, Dept Obstet & Gynecol, Chongqing, Peoples R China; [Otoo, Antonia; Czika, Armin; Lamptey, Jones; Yang, Jun-Pu; Wang, Mei-Jiao; Wang, Ying-Xiong; Ding, Yu-Bin] Chongqing Med Univ, Sch Publ Hlth, Dept Reprod Biol, Joint Int Res Lab Reprod & Dev, Chongqing, Peoples R China; [Czika, Armin] Transilvania Univ Brasov, Res & Dev Inst, Fac Med, Brasov, Romania; [Feng, Qian] Chongqing Hosp Tradit Chinese Med, Dept Gynecol, Chongqing, Peoples R China; [Wang, Mei-Jiao] Chongqing Med Univ, Sch Basic Med Sci, Dept Physiol, Chongqing, Peoples R China; [Ding, Yu-Bin] Changsha Med Univ, Acad Workstn, Dept Pharmacol, Changsha, Peoples R China"

通信作者："Wang, YX; Ding, YB (通讯作者)，Chongqing Med Univ, Women & Childrens Hosp, Dept Obstet & Gynecol, Chongqing, Peoples R China.; Wang, MJ; Wang, YX; Ding, YB (通讯作者)，Chongqing Med Univ, Sch Publ Hlth, Dept Reprod Biol, Joint Int Res Lab Reprod & Dev, Chongqing, Peoples R China.; Wang, MJ (通讯作者)，Chongqing Med Univ, Sch Basic Med Sci, Dept Physiol, Chongqing, Peoples R China.; Ding, YB (通讯作者)，Changsha Med Univ, Acad Workstn, Dept Pharmacol, Changsha, Peoples R China."

来源：REPRODUCTION

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001094078100003

JCR分区：Q1

影响因子：3.7

年份：2023

卷号：166

期号：5

开始页：323

结束页：336

文献类型：Article

关键词：　

摘要："PCOS is a reproductive disorder with an unclear etiology. It affects 5-10% of women worldwide and is largely associated with impaired glucose metabolism and obesity. HMGB1 is a nuclear protein associated with impaired glucose metabolism and PCOS. We sought to investigate the potential therapeutic effects of emodin on glucose metabolism and ovarian functions in PCOS mice via the HMGB1 molecular pathway. A high-fat diet (HFD) and dehydroepiandrosterone (DHEA)- induced PCOS mouse model comprising four experimental groups was established: control, PCOS, PCOS plus emodin, and PCOS plus vehicle groups. Emodin administration attenuated obesity, elevated fasting glucose levels, impaired glucose tolerance, and insulin resistance, and improved the polycystic ovarian morphology of PCOS mice. Additionally, it lowered elevated serum HMGB1, LH, and testosterone levels in PCOS mice. Elevated ovarian protein and mRNA levels of HMGB1 and TLR4 in PCOS mice were also lowered following emodin treatment. Furthermore, emodin lowered high NF-kappa B/65 protein levels in the ovaries of PCOS mice. Immunohistochemical staining of the ovaries revealed strong HMGB1, TLR4, and AR expressions in PCOS mice, which were lowered by emodin treatment. Moreover, emodin significantly increased GLUT4, IRS2, and INSR levels that were lowered by PCOS. Overall, our study showed that emodin alleviated the impaired glucose metabolism and improved ovarian function in PCOS mice, possibly via the HMGB1/TLR4/NF-kappa B signaling pathway. Thus, emodin could be considered a potential therapeutic agent in the management of PCOS."

基金机构："The authors would like to thank Sadaf Pervaz, Amin Ullah, and Su Yanan for their help during the sacrifice of mice and tissue processing."

基金资助正文："The authors would like to thank Sadaf Pervaz, Amin Ullah, and Su Yanan for their help during the sacrifice of mice and tissue processing."