MXene-incorporated C60NPs and Au@Pt with dual-electric signal outputs for accurate detection of<i> Mycobacterium</i><i> tuberculosis</i> ESAT-6 antigen

作者全名："Huang, He; Chen, Yuhan; Zuo, Jianli; Deng, Can; Fan, Junling; Bai, Lijuan; Guo, Shuliang"

作者地址："[Huang, He; Chen, Yuhan; Fan, Junling; Guo, Shuliang] Chongqing Med Univ, Affiliated Hosp 1, Dept Resp & Crit Care Med, Chongqing 400016, Peoples R China; [Zuo, Jianli; Bai, Lijuan] Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China; [Deng, Can] Chongqing Med Univ, Affiliated Hosp 1, Dept Hematol, Chongqing 400016, Peoples R China"

通信作者："Guo, SL (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Resp & Crit Care Med, Chongqing 400016, Peoples R China.; Bai, LJ (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China."

来源：BIOSENSORS & BIOELECTRONICS

ESI学科分类：CHEMISTRY

WOS号：WOS:001094495800001

JCR分区：Q1

影响因子：12.6

年份：2023

卷号：242

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：MXene-based nanohybrid; Dual-electric signal; Tuberculosis diagnosis; Electrochemical aptasensor

摘要："Rapid and effective detection of Mycobacterium tuberculosis (MTB) is the crux of minimizing tuberculosis (TB) spread. Consequently, a new electrochemical aptasensor based on dual-signal output for ultrasensitive detection of MTB early secreted antigenic target 6 (ESAT-6) antigen was developed. Especially, a new nanocomposite MXene/C60NPs/Au@Pt was synthesized for signal generation and amplification. In this biosensing architecture, dual independent signal outputs were achieved by coupling the electrochemical redox activity of fullerene nanoparticles (C60NPs) with the effective electrocatalytic activity of Au@Pt nanoparticles. MXene possesses a large specific surface area, allowing densely loaded of these two electroactive materials, further improved sensing capability. In addition, specific ESAT-6 antigen binding aptamers were attached to Au@Pt to create the tracer label. With a typical sandwich format along with the introduction of the gold nanoparticle-loaded molybdenum disulfide (MoS2-Au) as the sensing interface, the limit of detection (LOD) of the proposed aptasensor was 2.88 fg mL-1 (DPV measurement) and 13.50 fg mL-1 (IT measurement), respectively, with a broad linear range of 100 fg mL-1 to 50 ng mL-1. Significantly, it exhibited better specificity and accuracy with a sensitivity of 97.5% and a specificity of 96.7% to distinguish healthy donors, other lung diseases and TB patients compared to commercial ELISA assay, holding a promising prospect in clinical diagnosis."

基金机构："National Natural Science Foundation of China [82072378, 81601856]; National Major Science and Technology Projects of China [2018ZX10302302003]; Chongqing Talents: Exceptional Young Talents Project, China [CQYC202005015, cstc2021ycjh-bgzxm0328]; Ba Yu Scholar Program, China [YS2019020]; Chongqing talents projects-Famous masters and teachers; Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China (2019); Cultivation Foundation of the First Affiliated Hospital of Chongqing Medical University [499PYJJ2021-10]; First-rate Discipline Construction Project of First Clinical College Clinical Medicine of Chongqing Medical University [472020320230017]; Program for Youth Innovation in Future Medicine, Chongqing Medical University [W0119]; First-class Discipline Construction Project of First Clinical College Clinical Medicine of Chongqing Medical University [472020320230017]; Natural Science Foundation [CSTB2023NSCO-BHX0116]; Projects of Chongqing postdoctoral research [2022COBSHTB3068]"

基金资助正文："This work is supported by National Natural Science Foundation of China (82072378, 81601856) , the National Major Science and Tech-nology Projects of China (2018ZX10302302003) , Chongqing Talents: Exceptional Young Talents Project, China (CQYC202005015, cstc2021ycjh-bgzxm0328) , Chongqing talents projects-Famous masters and teachers (Shuliang Guo) , Ba Yu Scholar Program, China (YS2019020) , Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China (2019) , Cultivation Foundation of the First Affiliated Hospital of Chongqing Medical University (499PYJJ2021-10) , First-rate Discipline Construction Project of First Clinical College Clinical Medicine of Chongqing Medical University (472020320230017) , Program for Youth Innovation in Future Medicine, Chongqing Medical University (W0119) , First-class Discipline Construction Project of First Clinical College Clinical Medicine of Chongqing Medical University (472020320230017) , Natural Science Foundation (CSTB2023NSCO-BHX0116) , and Projects of Chongqing postdoctoral research (2022COBSHTB3068) ."