IGF2BP2 promotes head and neck squamous carcinoma cell proliferation and growth via the miR-98-5p/PI3K/Akt signaling pathway

作者全名："Yu, Dan; Xiao, Zhenlong; Zou, Zhefei; Lin, Ling; Li, Jing; Tan, Jian; Chen, Wei"

作者地址："[Yu, Dan; Zou, Zhefei; Lin, Ling; Li, Jing; Tan, Jian; Chen, Wei] Huazhong Univ Sci & Technol, Cent Hosp Wuhan, Tongji Med Coll, Dept Otorhinolaryngol, Wuhan, Peoples R China; [Xiao, Zhenlong] Hubei Univ Med, Cent Hosp Wuhan, Dept Otorhinolaryngol, Dept Obstet, Shiyan, Hubei, Peoples R China; [Chen, Wei] Huazhong Univ Sci & Technol, Cent Hosp Wuhan, Tongji Med Coll, Key Lab Mol Diag Hubei Prov, Wuhan, Peoples R China; [Chen, Wei] Chongqing Med Univ, State Key Lab Ultrasound Med & Engn, Chongqing, Peoples R China"

通信作者："Chen, W (通讯作者)，Huazhong Univ Sci & Technol, Cent Hosp Wuhan, Tongji Med Coll, Dept Otorhinolaryngol, Wuhan, Peoples R China.; Chen, W (通讯作者)，Huazhong Univ Sci & Technol, Cent Hosp Wuhan, Tongji Med Coll, Key Lab Mol Diag Hubei Prov, Wuhan, Peoples R China.; Chen, W (通讯作者)，Chongqing Med Univ, State Key Lab Ultrasound Med & Engn, Chongqing, Peoples R China."

来源：FRONTIERS IN ONCOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001094843600001

JCR分区：Q2

影响因子：3.5

年份：2023

卷号：13

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：IGF2BP2; HNSCC; tumorigenesis; miR-98-5p; PI3K/AKT

摘要："IntroductionAs a N6-methyladenosine reader protein, Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) is a critical player in tumor progression and metastasis. However, its specific function in head and neck squamous carcinoma (HNSCC) has yet to be determined. The present study aimed to determine the role of IGF2BP2 in HNSCC.MethodsThe expression of IGF2BP2 in HNSCC was analyzed using The Cancer Genome Atlas (TCGA) dataset and detected in HNSCC tissues and cells, respectively. Gain- and loss- of function methods were employed to study the effects of IGF2BP2 on HNSCC cell proliferation and tumorigenesis in vitro and in vivo. MicroRNAs (miRNAs) regulating IGF2BP2 were predicted using online tools and confirmed experimentally.ResultsWe showed augmented IGF2BP2 expression in HNSCC, which correlated with poor clinical outcomes. Functional studies showed that IGF2BP2 promoted HNSCC cell proliferation by facilitating cell cycle progression while inhibiting apoptosis. We further demonstrated that IGF2BP2 could enhance HNSCC cell tumorigenesis in vivo. Mechanistically, our data revealed that miR-98-5p could directly target IGF2BP2. The interplay between IGF2BP2 and miR-98-5p is essential to drive the progression of HNSCC via the phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)-protein kinase B (Akt) pathway signaling pathway.DiscussionThe current study revealed the oncogenic role of IGF2BP2 and provided insights into its potential mechanism in HNSCC tumorigenesis. Additionally, IGF2BP2 might represent a promising therapeutic target and serve as prognostic biomarker in patients with HNSCC."

基金机构："The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This project is supported by the Science and Technology Program of Wuhan Technology Bureau (2023020201020537). [2023020201020537]; Science and Technology Program of Wuhan Technology Bureau"

基金资助正文："We express our sincere gratitude to all team members for their invaluable assistance with this endeavor.r The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This project is supported by the Science and Technology Program of Wuhan Technology Bureau (2023020201020537)."