Aging-related genes revealed Neuroinflammatory mechanisms in ischemic stroke by bioinformatics

作者全名："Yao, Zhengyu; Jiang, Jin; Ju, Yaxin; Luo, Yong"

作者地址："[Yao, Zhengyu; Jiang, Jin; Ju, Yaxin; Luo, Yong] Chongqing Med Univ, Affiliated Hosp 1, Dept Neurol, Chongqing 400016, Peoples R China; [Yao, Zhengyu; Jiang, Jin; Ju, Yaxin; Luo, Yong] Chongqing Med Univ, Affiliated Hosp 1, Lab Res Ctr, Chongqing 400016, Peoples R China"

通信作者："Luo, Y (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Neurol, Chongqing 400016, Peoples R China."

来源：HELIYON

ESI学科分类：　

WOS号：WOS:001101269700001

JCR分区：Q1

影响因子：3.4

年份：2023

卷号：9

期号：11

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Ischemic stroke (IS) is a leading cause of disability, morbidity, and mortality globally. Aging affects immune function and contributes to poor outcomes of IS in elderly individuals. However, little is known about how aging-related genes (ARGs) are involved in IS. In this study, the relationship between ARGs and IS immune microenvironment biomarkers was explored by bioinformatics. Two IS microarray datasets (GSE22255, GSE16561) from human blood samples were analyzed and 502 ARGs were identified, from which 29 differentially expressed ARGs were selected. Functional analysis revealed that 7 of these ARGs (IL1B, FOS, JUN, CXCL5, PTGS2, TNFAIP3 and TLR4) were involved in five top enriched pathways (IL-17 signaling pathway, TNF signaling pathway, Rheumatoid arthritis, NF-kappa B signaling pathway and Pertussis) related to immune responses and inflammation. Five hub DE-ARGs (IL2RB, FOS, IL7R, ALDH2 and BIRC2) were identified using machine learning algorithms, and their asso-ciation with immune-related characteristics was confirmed by additional tests. Single-cell sequencing dataset GSE129788 was retrieved to analyze aging molecular-related features, which was in accordance with microarray datasets. Clustering analysis revealed two subtypes of IS, which were distinguished by their differential expression of genes related to the NF-kappa B signaling pathway. These findings highlight the importance of ARGs in regulating immune responses in IS and suggest potential prevention and treatment strategies as well as guidelines for future research."

基金机构：National Natural Science Foundation of China [30470606]; Postdoctoral Training ProgramFoundation of the First Affiliated Hospital of Chongqing Medical University [CYYY-BSHPYXM-202214]; China Postdoctoral Science Foundation [2022MD723736]

基金资助正文："This study was funded by the National Natural Science Foundation of China (Grant No. 30470606) , the Postdoctoral Training ProgramFoundation of the First Affiliated Hospital of Chongqing Medical University (Grand NO. CYYY-BSHPYXM-202214) and the China Postdoctoral Science Foundation (Grand NO. 2022MD723736) ."