A novel sandwich-type electrochemical immunosensor for sensitive detection of zearalenone using NG/PDDA/HNTs and Ti-MOF-KB composites for signal amplification

作者全名："Jiang, Xiaodan; Mu, Zhaode; Wang, Jie; Zhou, Jing; Bai, Lijuan"

作者地址："[Jiang, Xiaodan; Zhou, Jing; Bai, Lijuan] Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China; [Mu, Zhaode; Wang, Jie] Chongqing Med Univ, Coll Pharm, Res Ctr Pharmacodynam Evaluat Engn Technol Chongqi, Chongqing 400016, Peoples R China"

通信作者："Bai, LJ (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China."

来源：FOOD CHEMISTRY

ESI学科分类：AGRICULTURAL SCIENCES

WOS号：WOS:001101629800001

JCR分区：Q1

影响因子：8.5

年份：2024

卷号：436

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Mycotoxin; Ti-MOF-KB; Tb/NG/PDDA/HNTs; Immunosensor; Zearalenone (ZEA); Corn flour

摘要："In this work, a novel sandwich-type electrochemical immunosensor based on signal amplification was developed for the ultrasensitive detection of zearalenone (ZEA). The composite consisting of poly(diallyldimethylammonium chloride) functionalized nitrogen doped graphene, halloysite nanotubes and toluidine blue (Tb/NG/PDDA/HNTs), was synthesized for the first time. Then it was modified with gold nanoparticles (AuNPs) to bind the secondary antibody (Ab(2), 10 mu g mL(-1)) and form the tracer label. In addition, ketjen black (KB) was doped into titanium-based metal-organic framework (Ti-MOF), which provided large specific surface area and employed as the sensing platform to increase the immobilization of the primary antibody (Ab(1), 10 mu g mL(-1)). This immunosensor showed a wide linear range for ZEA from 10 fg mL(-1) to 100 ng mL(-1) with a limit of detection (LOD) as low as 0.57 fg mL(-1), which was below the maximum tolerable levels (50 similar to 100 mu g kg(-1)) set by the United Nations Food and Agriculture Organization (FAO)."

基金机构："National Natural Science Foundation of China [82072378, 81601856]; Chongqing Talents: Exceptional Young Talents Project, China [CQYC202005015, cstc2021ycjh-bgzxm0328]; Natural Science Founda-tion of Chongqing, China [2021XM2045]; Special Funding for Postdoctoral Research Project of Chongqing, China [W0067]; Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China; Program for Youth Innovation in Future Medicine, Chongqing Medical University, China [YXY2019XSGG4]; Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China [YXY2021BSH04, YS2019020]; Ba Yu Scholar Program, China; [CSTB2022NSCQ-BHX0006]"

基金资助正文："This work is supported by National Natural Science Foundation of China (82072378, 81601856) , Chongqing Talents: Exceptional Young Talents Project, China (CQYC202005015, cstc2021ycjh-bgzxm0328) , Ba Yu Scholar Program, China (YS2019020) , Natural Science Foundation of Chongqing, China (CSTB2022NSCQ-BHX0006) , Special Funding for Postdoctoral Research Project of Chongqing, China (2021XM2045) , Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China (2019) , Program for Youth Innovation in Future Medicine, Chongqing Medical University, China (W0067) and Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China (YXY2019XSGG4, YXY2021BSH04)."