N-acetylcysteine attenuates the incidence of phlebitis induced by carbomer/vinorelbine gel

作者全名："Zhang, Hongyu; Gong, Jing; Zhang, Shiyu; Luo, Liwen; Luo, Chengqin; Bi, Ke; Wang, Lei; Kan, Xuewei; Tian, Zhiqian; Wang, Xiaolong"

作者地址："[Zhang, Hongyu; Gong, Jing; Luo, Chengqin; Bi, Ke; Wang, Lei; Wang, Xiaolong] Chongqing Med Univ, Affiliated Hosp 2, Dept Emergency, Chongqing, Peoples R China; [Zhang, Shiyu; Tian, Zhiqian] Third Mil Med Univ, Army Med Univ, Inst Immunol, PLA, Chongqing, Peoples R China; [Luo, Liwen] Third Mil Med Univ, Army Med Univ, Xinqiao Hosp, Dept Orthopaed, Chongqing, Peoples R China; [Kan, Xuewei] Univ Sci & Technol China, Affiliated Hosp USTC 1, Dept Dermatol, Div Life Sci & Med, Hefei, Anhui, Peoples R China; [Tian, Zhiqian] Army Med Univ, Third Mil Med Univ, Inst Immunol, PLA, 30 Gaotanyan St, Chongqing 400038, Peoples R China; [Wang, Xiaolong] Chongqing Med Univ, Affiliated Hosp 2, Dept Emergency, 76 Linjiang Rd, Chongqing 400000, Peoples R China"

通信作者："Tian, ZQ (通讯作者)，Army Med Univ, Third Mil Med Univ, Inst Immunol, PLA, 30 Gaotanyan St, Chongqing 400038, Peoples R China.; Wang, XL (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Emergency, 76 Linjiang Rd, Chongqing 400000, Peoples R China."

来源：HELIYON

ESI学科分类：　

WOS号：WOS:001102332500001

JCR分区：Q1

影响因子：3.4

年份：2023

卷号：9

期号：11

开始页：　

结束页：　

文献类型：Article

关键词：Phlebitis; Carbomer; N-acetylcysteine; Vinorelbine; Sustained-release carrier gel

摘要："Background: The high incidence and severe clinical manifestations of phlebitis pose a complex and urgent clinical challenge. The rapid and simple establishment of animal phlebitis models and the development of preventive strategies are crucial to resolving this problem.Methods: In this study, we established such models by mixing vinorelbine ditartrate (VNR) and carbomer to form a sustained-release gel carrier, and then injected it around the veins rather than inside the vessels. Furthermore, we analyzed the efficacy of the carbomer/VNR gel in inducing phlebitis by monitoring the morphology of the veins using HE staining, immunohistochemical and immunofluorescence staining, and western blotting. Reactive oxygen species (ROS) and lipid peroxidation levels were determined using flow cytometry. Finally, we evaluated the inhibitory effect of N-acetylcysteine (NAC) on VNR-induced phlebitis in rabbits and rats. Results: Our findings suggested that the carbomer/VNR gel rapidly and easily induced phlebitis due to by retention of the gel in situ, wrapping the veins, and the prolonged release of VNR. NAC alleviated the VNR-induced oxidative stress response and expression of inflammatory cytokines by attenuating mitochondrial damage in venous endothelial cells, thereby preventing the occurrence of phlebitis in rabbits and rats.Conclusion: The in situ carbomer/VNR gel provides a rapid and simple method for establishing an animal model to study the pathogenesis of phlebitis. Furthermore, the observed therapeutic effect of NAC highlights its novel and efficacious role in preventing and treating phlebitis."

基金机构：Nanan District Science and Health Joint Medical Research Project Fund [2021-06]

基金资助正文：We would like to thank the Army Medical University Animal Center for providing laboratory animals and Editage ( www.editage. cn) for English language editing. This work was supported by the Nanan District Science and Health Joint Medical Research Project Fund (Grant Number: 2021-06) .