Construction of a mitochondria-targeted near-infrared fluorescence turn-on fluorescent probe for H2S detection and imaging in living cells and drug-induced mice inflammatory models

作者全名："Liu, Xiang; Lei, Haibo; Hu, Yixiang; Zou, Xinrong; Ran, Hongyan; Cai, Qinuo; Huang, Jianji; Liu, Chang"

作者地址："[Liu, Xiang; Lei, Haibo; Hu, Yixiang; Liu, Chang] Xiangtan Cent Hosp, Dept Clin Pharm, Xiangtan 411100, Peoples R China; [Zou, Xinrong; Ran, Hongyan; Cai, Qinuo; Huang, Jianji] Chongqing Med Univ, Coll Pharm, Chongqing 400016, Peoples R China"

通信作者："Liu, X (通讯作者)，Xiangtan Cent Hosp, Dept Clin Pharm, Xiangtan 411100, Peoples R China."

来源：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

ESI学科分类：CHEMISTRY

WOS号：WOS:001102579000001

JCR分区：Q1

影响因子：4.3

年份：2024

卷号：306

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Signaling molecule; Hydrogen sulfide; Mitochondrial-targeting; Inflammatory; Drug-induced

摘要："The mechanism of the interaction between the signaling molecule hydrogen sulfide (H2S) and mitochondria and its related diseases is difficult to elusive. Thus it is urgent to develop effective methods and tools to visualize H2S in mitochondria and in vivo. In this work, a robust mitochondrial-targeting NIR fluorescence ""turn-on"" fluorescent probe, NIR1, was reported, by adopting a Changsha-OH near-infrared (NIR) dye as the NIR fluorophore, a 2,4-dinitrophenyl (DNB) moiety as both the responsive site of the H2S and the fluorescence quenching group of the NIR fluorophore, and an oxygen onium ion site as the mitochondria-targeting group, for the detection and analysis of H2S in living Raw 264.7 cells and drug-induced inflammatory mice models. NIR1 exhibited a much smaller background fluorescence signal in lack of H2S, whereas strong enhanced NIR fluorescence ""turn-on"" was detected in the presence of H2S, these results showed a low detection limit (30.2 nM) for quantitative detection of H2S in aqueous solutions with concentrations ranging from 0 to 1 mu M H2S. These characteristics were beneficial to direct detection and imaging analysis of H2S in complicated biosystems. Therefore, first, NIR1 was applied for the NIR detection of mitochondrial H2S in living inflammatory cells with satisfactory results. Finally, NIR1 was applied to detect H2S in drug-induced inflammatory mice models with agreeable results, demonstrating that NIR1 as a molecular tool has an excellent practical application in the study of the interaction between inflammatory and H2S."

基金机构：Hunan Pharmaceutical Society [HN201707]; Health Commission of Hunan Province [B2017167]

基金资助正文：<B>Acknowledgements</B> This work was partially supported by Hunan Pharmaceutical Society (HN201707) and The Health Commission of Hunan Province (B2017167) .