Astragaloside improves asthmatic airway inflammation mediated by inhibition of early growth response-1 through S14G-humanin

作者全名："Zhou, Shengnan; Li, Youlun"

作者地址："[Zhou, Shengnan; Li, Youlun] Chongqing Med Univ, Affiliated Hosp 1, Dept Resp & Crit Care Med, Chongqing 400016, Peoples R China"

通信作者："Li, YL (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Resp & Crit Care Med, Chongqing 400016, Peoples R China."

来源：MATERIALS EXPRESS

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001108726800007

JCR分区：Q4

影响因子：0.7

年份：2023

卷号：13

期号：9

开始页：1512

结束页：1518

文献类型：Article

关键词：Astragaloside; S14G-Humanin; Egr-1; Asthmatic Airway Inflammation; Rat Bronchial Smooth Muscle Cells

摘要："In this experiment, we explored the role of astragaloside in regulating Egr-1 through S14G-humanin on asthmatic airway inflammation. 64 juvenile Sprague Dawley (SD) rats were selected. After establishing rat asthma model, they were assigned into blank control group, astragaloside group, S14G-Humanin group and astragaloside+S14G-Humanin group (combined group). Astragaloside group was intervened with astragaloside II 0.6 mg/kg, S14G-Humanin group was intervened with 50 mu m S14G-Humanin, combined group IP: 203.8 109 20 On Sat, 07 Oct 2023 05 29:21 RBSMCs were treated with astragaloside II 0.6 mg/kg and 50 mu M S14G-Humaninn. Airway responsiveness was assessed and pathological damage of lung tissue was assessed by HE staining along with analysis of Delivered by Ingenta inflammatory cells in bronchoalveolar lavage fluid (BALF), inflammatory cytokines and bone-marrow derived mesenchymal stem cells (rBMSCs) behaviors. Compared to blank control, the Penh values of astragaloside group, S14G-Humanin group and combination group were increased (P < 0.05) and pathological scores were lower with the lowest score in combined group (all P < 0.05). The number of white blood cells, neutrophils, eosinophils, macrophages and lymphocytes in BALF of rats in astragaloside group, S14G-Humanin group and combination group were decreased, with the lowest number in combination group (P < 0.05). In addition, IL-4, IL-6, and IL-21 in astragaloside group, S14G-Humanin group and combination group were reduced, with the lowest levels in combination group (P < 0.05). RBSMCs proliferation and migration ability in treatment group was reduced with the lowest in combination group (P < 0.05). After up-regulating S14G-Humanin, Egr1 mRNA expression was elevated (P < 0.05). Astragaloside can reduce inflammatory cells and inflammatory cytokines and increase the expression of Egr-1 by regulating S14G-Humanin expression."

基金机构：　

基金资助正文：