Pyrylium-based derivatization for rapid labeling and enhanced detection of thiol in mass spectrometry imaging

作者全名："Huang, Shuai; Wang, Haiyang; Liu, Xinxin; Liu, Lanxiang; Liu, Dan; Zhang, Xiaozhe; Zhang, Lihua; Xie, Peng; Zhang, Yukui"

作者地址："[Huang, Shuai; Liu, Xinxin; Liu, Dan; Zhang, Xiaozhe; Zhang, Lihua; Zhang, Yukui] Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China; [Huang, Shuai] Univ Chinese Acad Sci, Beijing 100039, Peoples R China; [Wang, Haiyang; Liu, Lanxiang; Xie, Peng] Chongqing Med Univ, Chongqing 400016, Peoples R China"

通信作者："Liu, D; Zhang, XZ (通讯作者)，Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China."

来源：ANALYTICA CHIMICA ACTA

ESI学科分类：CHEMISTRY

WOS号：WOS:001109348600001

JCR分区：Q1

影响因子：5.7

年份：2023

卷号：1284

期号：　

开始页：　

结束页：　

文献类型：Article

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摘要："Many endogenous antioxidants, including glutathione (GSH), cysteine (Cys), cysteinyl-glycine (Cys-Gly) and homocysteine (Hcy) possess free thiol functional groups. In most cases, matrix-assisted laser desorption ionization (MALDI) analyses of trace amounts of thiol compounds are challenging because of their instability and poor ionization properties. We present a mass spectrometry imaging (MSI) approach for mapping of thiol compounds on brain tissue sections. Our derivatization reagents 1-(2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl) ethyl)-2,4,6-trimethylpyridinium (MTMP) and 1-(2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)-2,4,5-triphenylpyridinium (MTPP) facilitate the covalent charge-tagging of molecules containing free thiol group for the selective and rapid detection of GSH synthesis and metabolic pathway related metabolites by MALDI-MSI. The developed thiol-specific mass spectrometry imaging method realizes the quantitative detection of exogenous Nacetylcysteine tissue sections, and the detection limit in mass spectrometry imaging could reach 0.05 ng. We illustrate the capabilities of the developed method to mapping of thiol compounds on brain tissue from the chronic social defeat stress (CSDS) depression model mice."

基金机构："Dalian Institute of Chemical Physics (DICP) [I202207]; Chinese Academy of Sciences (CAS) [121421-KYSB20200006]; Guizhou Department of Science and Technology [[2020] 4Y218]; Liaoning Department of Science and Technology [2021JH2, 10200004]"

基金资助正文："The research was funded by Dalian Institute of Chemical Physics (DICP I202207) , Chinese Academy of Sciences (CAS 121421-KYSB20200006) , Guizhou Department of Science and Technology ( [2020] 4Y218) ; Liaoning Department of Science and Technology (2021JH2/10200004) ."