The Nrf2 Pathway Alleviates Overloading Force-Induced TMJ Degeneration by Downregulating Oxidative Stress Reactions

作者全名："Xu, Minglu; Fang, Lingli; Xue, Qin; Zhang, Xuyang; He, Yao"

作者地址："[Xu, Minglu; Fang, Lingli; Xue, Qin; Zhang, Xuyang; He, Yao] Chongqing Med Univ, Stomatol Hosp, Dept Orthodont, 426 North Songshi Ave, Chongqing 401147, Peoples R China; [Xu, Minglu; Fang, Lingli; Xue, Qin; Zhang, Xuyang] Chongqing Med Univ, Stomatol Hosp, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [He, Yao] Chongqing Med Univ, Stomatol Hosp, Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China"

通信作者："He, Y (通讯作者)，Chongqing Med Univ, Stomatol Hosp, Dept Orthodont, 426 North Songshi Ave, Chongqing 401147, Peoples R China."

来源：JOURNAL OF INFLAMMATION RESEARCH

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001112223900001

JCR分区：Q2

影响因子：4.2

年份：2023

卷号：16

期号：　

开始页：5601

结束页：5612

文献类型：Article

关键词：NF-E2-related factor 2; oxidative stress; temporomandibular joint; cartilage; mechanical force

摘要："Objective: Oxidative stress is involved in the mechanisms associated with temporomandibular joint (TMJ) diseases. Nuclear factor erythroid 2-related factor 2 (Nrf2) is a crucial oxidative stress marker, but the specific mechanisms of its regulation in the early stages of mandibular condylar cartilage (MCC) degeneration remain unclear. This study aimed to explore the regulatory role of Nrf2 and its related oxidative stress signaling pathway in the early stage of MCC degeneration.Materials and Methods: Overloading force-induced MCC degeneration was performed in wild-type and Nrf2 knockout mice, as well as in mice after treatment with the Nrf2 activator cardamonin. Changes in MCC degeneration and the expression of oxidative stress markers in the corresponding situations were observed.Results: Nrf2 and NADPH oxidase 2 (NOX2) expression were elevated during early MCC degeneration induced by an overloading force. MCC degeneration was aggravated when Nrf2 was knocked out, accompanied by increased NOX2 and superoxide dismutase 2 (SOD2) expression. The MCC degeneration process was alleviated after cardamonin treatment, with activation of the Nrf2 pathway and decreased NOX2 and SOD2 expression.Conclusion: Early MCC degeneration is accompanied by mild oxidative stress progression. Activated Nrf2 and related pathways could alleviate the degeneration of MCC."

基金机构：China Postdoctoral Science Foundation [2018M640902]

基金资助正文：Funding This work was supported by China Postdoctoral Science Foundation (2018M640902 to Y.H.) .