Movable toehold for leakless self-assembly circuits

作者全名："Han, Xiaole; Yu, Hongyan; Zhang, Li; Weng, Zhi; Dai, Ling; Wang, Li; Song, Lin; Wang, Zhongzhong; Zhao, Rong; Wang, Luojia; Wang, Weitao; Bai, Dan; Guo, Yongcan; Lv, Ke; Xie, Guoming"

作者地址："[Han, Xiaole; Yu, Hongyan; Zhang, Li; Weng, Zhi; Dai, Ling; Song, Lin; Wang, Zhongzhong; Zhao, Rong; Wang, Luojia; Wang, Weitao; Bai, Dan; Xie, Guoming] Chongqing Med Univ, Key Lab Clin Lab Diag, Chinese Minist Educ, Coll Lab Med,Chongqing Med Lab Microfluid, Chongqing 400016, Peoples R China; [Han, Xiaole; Yu, Hongyan; Zhang, Li; Weng, Zhi; Dai, Ling; Song, Lin; Wang, Zhongzhong; Zhao, Rong; Wang, Luojia; Wang, Weitao; Bai, Dan; Xie, Guoming] Chongqing Med Univ, SPRi Engn Res Ctr, Chongqing 400016, Peoples R China; [Wang, Li] Chongqing Med Univ, Affiliated Hosp 1, Ctr Clin Mol Med Detect, Chongqing 400016, Peoples R China; [Guo, Yongcan] Southwest Med Univ, LuZhou Key Lab Nanobiosensing & Microfluid Point C, Clin Lab, Tradit Chinese Med Hosp, Luzhou 646000, Peoples R China; [Lv, Ke] Chongqing Med Univ, Affiliated Hosp 1, Dept Neurosurg, Chongqing 400016, Peoples R China"

通信作者："Xie, GM (通讯作者)，Chongqing Med Univ, Key Lab Clin Lab Diag, Chinese Minist Educ, Coll Lab Med,Chongqing Med Lab Microfluid, Chongqing 400016, Peoples R China.; Xie, GM (通讯作者)，Chongqing Med Univ, SPRi Engn Res Ctr, Chongqing 400016, Peoples R China.; Guo, YC (通讯作者)，Southwest Med Univ, LuZhou Key Lab Nanobiosensing & Microfluid Point C, Clin Lab, Tradit Chinese Med Hosp, Luzhou 646000, Peoples R China.; Lv, K (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Neurosurg, Chongqing 400016, Peoples R China."

来源：BIOSENSORS & BIOELECTRONICS

ESI学科分类：CHEMISTRY

WOS号：WOS:001113521700001

JCR分区：Q1

影响因子：10.7

年份：2024

卷号：245

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：　

摘要："Nonenzymatic self-assembly circuit utilizing hairpin substrates has been developed to be a powerful tool for information transduction, amplification and compu-tation. However, the sensitivity, stability and application of this circuit are impeded by the presence of leakage which refers to undesired triggering in the absence of input. Herein, we proposed a movable toehold principle to suppress leakage and accelerate the catalytic reaction through removing partial hairpin toehold responsible for the leakage and transferring it to the catalyst. With movable toehold, catalytic hairpin assembly (called mtCHA) exhibited an excellent signal-to -background ratio of over 100, high robustness and improved specificity. In more complex circuit, including proximity recognition, signal amplification of small molecules (such as ATP), logic network, autocatalysis circuit and two-layer cascade circuit, mtCHA also demonstrated satisfactory performance. Our findings suggest that mtCHA holds great potential for broader applications, and the approach of repurposing harmful fragments into beneficial candidates can provide valuable insights for other chemical systems."

基金机构："National Key R & D Program of China [2022YFC2603800]; National Natural Science Foundation of China [82,172,369, 82,201,633]; Graduate Scientific Research and Innovation Project of Chongqing [CYS22362]"

基金资助正文："This work was supported by the National Key R & D Program of China (2022YFC2603800) , the National Natural Science Foundation of China (82,172,369 and 82,201,633) and the Graduate Scientific Research and Innovation Project of Chongqing (CYS22362) ."