Identification of P3H1 as a Predictive Prognostic Biomarker for Bladder Urothelial Carcinoma Based on the Cancer Genome Atlas Database

作者全名："Zhang, Yuanfeng; Chen, Yang; Chen, Zhiming; Zhou, Xinye; Chen, Shaochuan; Lan, Kaijian; Wang, Zhiping; Zhang, Yonghai"

作者地址："[Zhang, Yuanfeng; Chen, Shaochuan; Lan, Kaijian; Zhang, Yonghai] Shantou Cent Hosp, Dept Urol, Shantou, Peoples R China; [Chen, Yang] Chongqing Med Univ, Dept Nursing, Bishan Hosp, Chongqing, Peoples R China; [Chen, Zhiming] Shantou Cent Hosp, Dept Pathol, Shantou, Peoples R China; [Zhou, Xinye] Shantou Cent Hosp, Ctr Reprod Med, Shantou, Peoples R China; [Wang, Zhiping] Lanzhou Univ, Hosp 2, Dept Urol, Lanzhou, Peoples R China"

通信作者："Zhang, YH (通讯作者)，Shantou Cent Hosp, Dept Urol, Shantou, Peoples R China."

来源：PHARMACOGENOMICS & PERSONALIZED MEDICINE

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:001115845800001

JCR分区：Q3

影响因子：1.9

年份：2023

卷号：16

期号：　

开始页：1041

结束页：1053

文献类型：Article

关键词：prolyl 3-hydroxylase 1; bladder cancer; immune infiltration; drug sensitivity

摘要："Purpose: The extracellular matrix in the tumor microenvironment are closely related to the development of tumors. This study's primary aim is to study the association between prolyl 3-hydroxylase 1 (P3H1) which mainly expresses collagen in extracellular matrix and the progression and prognosis of bladder cancer (BC).Methods: The clinical and transcriptome data were acquired from the cancer genome atlas database. BLCAsubtyping is used to evaluate tissue subtypes of BC. The COX proportional hazards can be used to evaluate the survival process's influencing factors. Immunohistochemistry was used to identify differences in the expression of P3H1 in cancer and paired adjacent tissues. GSEA was used to investigate the underlying biological processes. Finally, ssGSEA, TIMER and pRRophetic were used to study the relationship between P3H1 and immune cell infiltration and drug sensitivity. Results: The expression of P3H1 was substantially higher in highly invasive BC samples than in low invasive BC. P3H1 was an independent predictor of overall survival (HR = 1.12, p = 0.03). P3H1 expression was significantly higher in tumor tissues than adjacent normal tissues in clinical tissue samples, and was significantly higher in highly stage cancer than low stage cancer samples. Samples with high P3H1 expression had a higher level of immune cell infiltration and immune function, as well as a significant correlation with macrophage and dendritic cell infiltration and TGF-beta, Th1 cells, and macrophage regulation (cor >0.3, p <0.05). P3H1 high expression samples were substantially more sensitive to docetaxel, cisplatin, vinblastine, camptothecin, paclitaxel, and other medicines than P3H1 low expression samples.Discussion: P3H1 is a possible oncogene and an independent predictor of poor prognosis in BC; it also has enhanced sensitivity to docetaxel, cisplatin, vinblastine, camptothecin, paclitaxel, and other medications."

基金机构：National Natural Science Foundation of China [82372685]; Medical Science and Technology Research Fund Project of Guangdong Province of China [B2023489]

基金资助正文：Acknowledgments This work was supported by the National Natural Science Foundation of China (grant No.82372685) ; Medical Science and Technology Research Fund Project of Guangdong Province of China (grant No. B2023489) .