Nanobody-loaded nanobubbles targeting the G250 antigen with ultrasound/photoacoustic/fluorescence multimodal imaging capabilities for specifically enhanced imaging of RCC

作者全名："Chen, Jiajiu; Li, Jingyi; Zhong, Chengjie; Ling, Yi; Liu, Deng; Li, Xin; Xu, Jing; Liu, Qiuli; Guo, Yanli; Wang, Luofu"

作者地址："[Chen, Jiajiu; Li, Jingyi; Xu, Jing; Liu, Qiuli; Wang, Luofu] Army Med Univ, Daping Hosp, Dept Urol, Chongqing 400042, Peoples R China; [Zhong, Chengjie] Chongqing Med Univ, Clin Med Coll 2, Chongqing 400016, Peoples R China; [Ling, Yi; Liu, Deng; Li, Xin; Guo, Yanli] Army Med Univ, Southwest Hosp, Dept Ultrasound, Chongqing 400038, Peoples R China"

通信作者："Wang, LF (通讯作者)，Army Med Univ, Daping Hosp, Dept Urol, Chongqing 400042, Peoples R China.; Guo, YL (通讯作者)，Army Med Univ, Southwest Hosp, Dept Ultrasound, Chongqing 400038, Peoples R China."

来源：NANOSCALE

ESI学科分类：PHYSICS

WOS号：WOS:001115964900001

JCR分区：Q1

影响因子：5.8

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：　

摘要："Clinicians have attempted to discover a noninvasive, easy-to-perform, and accurate method to distinguish benign and malignant renal masses. The targeted nanobubbles (NBs) we constructed that target the specific membrane antigen of renal cell carcinoma (RCC), G250, and contain indocyanine green (ICG) provide multimodal enhanced imaging capability in ultrasound/photoacoustic/fluorescence for RCC which may possibly solve this problem. In this study, we encapsulated ICG in the lipid shell of the NBs by mechanical oscillation, then anti-G250 nanobodies (AGN) were coupled to the surfaces by the biotin-streptavidin bridge method, and the nanobubble named AGN/ICG-NB was completely constructed. The average particle diameter of the prepared AGN/ICG-NBs was (427.2 +/- 4.50) nm, and the zeta potential was (-13.33 +/- 1.01) mV. Immunofluorescence and flow cytometry confirmed the specific binding capability of AGN/ICG-NBs to G250-positive cells. In vitro imaging experiments confirmed the multimodal imaging capability of AGN/ICG-NBs, and the in vivo imaging experiments demonstrated the specifically enhanced ability of AGN/ICG-NBs for ultrasound/photoacoustic/fluorescence imaging of human-derived RCC tumors. The biosafety of AGN/ICG-NB was verified by CCK-8 assay, organ H&E staining and blood biochemical indices. In conclusion, the targeted nanobubbles we prepared with ultrasound/photoacoustic/fluorescence multimodal imaging capabilities provide a potentially feasible approach to address the need for early diagnosis and differential diagnosis of renal masses. Clinicians have attempted to discover a noninvasive, easy-to-perform, and accurate method to distinguish benign and malignant renal masses."

基金机构：Training Plan of Talent Innovation Ability of the Army Medical Center [2019CXLCB009]

基金资助正文：Many thanks to Professor Pan Li and his team from Chongqing Institute of Ultrasound Imaging for their support of the photoacoustic part of this study. This study was supported by the Training Plan of Talent Innovation Ability of the Army Medical Center (no. 2019CXLCB009).