"Upregulation of CELSR1 expression promotes ovarian cancer cell proliferation, migration, and invasion"

作者全名："Zuo, Jiwei; Zheng, Anqi; Wang, Xingyue; Luo, Zhicheng; Chen, Yueming; Cheng, Xiaoxiao; Zhao, Yuemei; Zhou, Xian; Tang, Kai-Fu; Du, Xing"

作者地址："[Zuo, Jiwei; Zheng, Anqi; Wang, Xingyue; Luo, Zhicheng; Chen, Yueming; Cheng, Xiaoxiao; Zhao, Yuemei; Tang, Kai-Fu] Wenzhou Med Univ, Affiliated Hosp 1, Key Lab Diag & Treatment Severe Hepatopancreat Dis, Wenzhou 325015, Zhejiang, Peoples R China; [Zhou, Xian] Chongqing Univ, Dept Radiat Oncol, Canc Hosp, 181 Hanyu Rd, Chongqing 400030, Peoples R China; [Tang, Kai-Fu] Chongqing Med Univ, Key Lab Mol Biol Infect Dis, Minist Educ, Chongqing 400016, Peoples R China; [Du, Xing] Chongqing Med Univ, Affiliated Hosp 1, Dept Orthoped, Chongqing 400016, Peoples R China"

通信作者："Tang, KF (通讯作者)，Wenzhou Med Univ, Affiliated Hosp 1, Key Lab Diag & Treatment Severe Hepatopancreat Dis, Wenzhou 325015, Zhejiang, Peoples R China.; Tang, KF (通讯作者)，Chongqing Med Univ, Key Lab Mol Biol Infect Dis, Minist Educ, Chongqing 400016, Peoples R China.; Du, X (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Orthoped, Chongqing 400016, Peoples R China."

来源：MEDICAL ONCOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001118300800001

JCR分区：Q2

影响因子：2.8

年份：2023

卷号：41

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：CELSR1; Ovarian cancer; Proliferation; RNA sequencing; Proteoglycans

摘要："Cadherin epidermal growth factor and laminin-G seven-pass G-type receptor 1 (CELSR1) is a planar cell polarity protein involved in the transmission of directional cues to align either individual cells within an epithelial sheet or multicellular clusters. CELSR1 has been suggested to play a role in glioma, breast cancer, and chronic lymphocytic leukemia development; however, whether it has a role in the pathogenesis of ovarian cancer remains unknown. The aim of this study was to determine the role of CELSR1 in ovarian cancer and elucidate its underlying molecular mechanisms. By analyzing gene expression data downloaded from the Cancer Genome Atlas database, we found that CELSR1 expression was upregulated in ovarian cancer tissues compared to that in normal ovarian tissues. High CELSR1 expression levels were associated with poor prognosis in patients with ovarian cancer. Cell proliferation, scratch, and transwell assays revealed that CELSR1 promoted the proliferation, migration, and invasion of ovarian cancer cells in vitro. In addition, transcriptome sequencing analysis revealed that CELSR1 knockdown in T29H cells resulted in the dysregulation of the expression of 1320 genes. Further analysis revealed that genes involved in proliferation- and migration-associated signaling pathways were regulated by CELSR1. Our study demonstrates that CELSR1 is highly expressed in ovarian cancer cells and regulates their proliferation and migration, suggesting its potential as a diagnostic marker and therapeutic target."

基金机构：National Natural Science Foundation of China

基金资助正文：None