A Spatially Controlled Proximity Split Tweezer Switch for Enhanced DNA Circuit Construction and Multifunctional Transduction

作者全名："Bai, Dan; Zhang, Jianhong; Zhang, Yaoyi; Yu, Hongyan; Zhang, Li; Han, Xiaole; Lv, Ke; Wang, Li; Luo, Wang; Wu, You; Zhou, Xi; Wang, Weitao; Feng, Tong; Xie, Guoming"

作者地址："[Bai, Dan; Zhang, Yaoyi; Yu, Hongyan; Han, Xiaole; Luo, Wang; Wu, You; Zhou, Xi; Wang, Weitao; Feng, Tong; Xie, Guoming] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Chongqing Med Lab Microfluid,Chinese Minist Educ, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China; [Bai, Dan; Zhang, Yaoyi; Yu, Hongyan; Han, Xiaole; Luo, Wang; Wu, You; Zhou, Xi; Wang, Weitao; Feng, Tong; Xie, Guoming] Chongqing Med Univ, SPRi Engn Res Ctr, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China; [Zhang, Jianhong] Chongqing Med Univ, Affiliated Hosp 1, Clin Labs, Chongqing 400016, Peoples R China; [Zhang, Li] Chongqing Med Univ, Dept Forens, Chongqing 400016, Peoples R China; [Lv, Ke] First Affiliated Hosp Chongqing Med Univ, Dept Neurosurg, Chongqing, Peoples R China; [Wang, Li] Chongqing Med Univ, Affiliated Hosp 1, Ctr Clin Mol Med Detect, Chongqing 400016, Peoples R China"

通信作者："Xie, GM (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Chongqing Med Lab Microfluid,Chinese Minist Educ, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China.; Xie, GM (通讯作者)，Chongqing Med Univ, SPRi Engn Res Ctr, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China."

来源：SMALL

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001118503400001

JCR分区：Q1

影响因子：13

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：associative toehold; DNA circuits; logic gates; nanotweezers; proximity effect

摘要："DNA strand displacement reactions are vital for constructing intricate nucleic acid circuits, owing to their programmability and predictability. However, the scarcity of effective methods for eliminating circuit leakages has hampered the construction of circuits with increased complexity. Herein, a versatile strategy is developed that relies on a spatially controlled proximity split tweezer (PST) switch to transduce the biomolecular signals into the independent oligonucleotides. Leveraging the double-stranded rigidity of the tweezer works synergistically with the hindering effect of the hairpin lock, effectively minimizing circuit leakage compared with sequence-level methods. In addition, the freely designed output strand is independent of the target binding sequence, allowing the PST switch conformation to be modulated by nucleic acids, small molecules, and proteins, exhibiting remarkable adaptability to a wide range of targets. Using this platform, established logical operations between different types of targets for multifunctional transduction are successfully established. Most importantly, the platform can be directly coupled with DNA catalytic circuits to further enhance transduction performance. The uniqueness of this platform lies in its design straightforwardness, flexibility, scalable intricacy, and system compatibility. These attributes pave a broad path toward nucleic acid-based development of sophisticated transduction networks, making them widely applied in basic science research and biomedical applications. A versatile spatially controlled proximity split tweezer (PST) switch for the transduction of diverse biomolecule into desired oligonucleotides is presented. Leveraging synergy between tweezer rigidity and hairpin lock can minimize DNA circuit leakage. The platform allows logical operations and direct coupling with DNA catalytic circuits. Its design simplicity, flexibility, and expandable complexity offer broad applications in research and biomedicine.image"

基金机构：National Natural Science Foundation of China; National Key R&D Program of China [2022YFC2603800]; Graduate Scientific Research and Innovation Project of Chongqing [CYB23215]; [82372351]; [82172369]; [82201633]; [8200225]

基金资助正文："D.B., J.Z., and Y.Z. contributed equally to this work. This work was financially supported by the National Key R&D Program of China (2022YFC2603800), the National Natural Science Foundation of China (82372351, 82172369, 82201633, 8200225), and the Graduate Scientific Research and Innovation Project of Chongqing (CYB23215)."