MicroRNA hsa-miR-320a-3p and Its Targeted mRNA FKBP5 Were Differentially Expressed in Patients with HIV/TB Co-Infection

作者全名："Li, Anlong; Bao, Jiajia; Gao, Sijia; He, Ying; Nie, Xiaoping; Hosyanto, Felycia Fernanda; He, Xintong; Li, Tongxin; Xu, Lei"

作者地址："[Li, Anlong; Bao, Jiajia; Gao, Sijia; He, Xintong; Xu, Lei] Chongqing Med Univ, Sch Basic Med, Dept Pathogen Biol, Chongqing 400016, Peoples R China; [Bao, Jiajia] First Peoples Hosp Jintang Cty, Hosp Acquired Infect Control Dept, Chengdu 610400, Peoples R China; [He, Ying; Nie, Xiaoping; Li, Tongxin] Southwest Univ, Chongqing Publ Hlth Med Ctr, Cent Lab, Publ Hlth Hosp, Chongqing 400036, Peoples R China; [Hosyanto, Felycia Fernanda] Chongqing Med Univ, Dept Clin Med, Chongqing 400016, Peoples R China"

通信作者："Xu, L (通讯作者)，Chongqing Med Univ, Sch Basic Med, Dept Pathogen Biol, Chongqing 400016, Peoples R China.; Li, TX (通讯作者)，Southwest Univ, Chongqing Publ Hlth Med Ctr, Cent Lab, Publ Hlth Hosp, Chongqing 400036, Peoples R China."

来源：ACS INFECTIOUS DISEASES

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:001119695600001

JCR分区：Q1

影响因子：4

年份：2023

卷号：9

期号：9

开始页：1742

结束页：1753

文献类型：Article

关键词：FKBP5; hsa-miR-320a-3p; HIV; tuberculosis; co-infection

摘要："Among the PLWH (people living with HIV) population, the risk of developing active tuberculosis (TB) is increasing. Active TB also accelerates the deterioration of PLWH's immune function and is one of the leading causes of death in the PLWH population. So far, accurate diagnosis of active TB in the PLWH population remains challenging. Through data analysis of HIV/TB co-infection in the GEO database, the differentially expressed genes as well as their related microRNA (miRNA) were acquired and were further verified through clinical blood samples. Dual-luciferase assay was used to verify the mechanism of miRNA on mRNA. The enrichment of immune cells in database patient samples was analyzed by bioinformatics and finally verified by blood routine data. Our study found that FKBP5 (FK506 binding protein 5) was highly expressed in the HIV/TB co-infection group; hsa-miR-320a-3p was highly expressed in the HIV infection group but decreased in the HIV/TB co-infection group. Dual-luciferase assay results showed that hsa-miR-320a-3p mimics significantly reduced the relative luciferase activity of the WT-FKBP5 group; however, this phenomenon was not observed in the MUT-FKBP5 group. At the same time, as a key molecule of the immune-related pathway, FKBP5 is highly correlated with the amount of neutrophils, which provides a new suggestion for the treatment of the HIV/TB co-infection population. Our study found that hsa-miR-320a-3p can decrease FKBP5 expression, suggesting a potential regulatory role for FKBP5. The involvement of FKBP5 and its related molecule hsa-miR-320a-3p in HIV/TB co-infection proposes them as potential biomarkers for the diagnosis of active TB in the PLWH population."

基金机构："Chongqing Municipal Science and Technology Commission [cstc2017jcyjAX0409]; CQMU Program for Youth Innovation in Future Medicine [W 0091]; Chongqing Medical Scientific Research Project (Joint Project of Chongqing Health Commission and Science and Technology Bureau) [2020FYYX001, 2022MSXM149]"

基金资助正文："This work was financially supported by the Chongqing Municipal Science and Technology Commission (no. cstc2017jcyjAX0409), CQMU Program for Youth Innovation in Future Medicine (W 0091), and Chongqing Medical Scientific Research Project (Joint Project of Chongqing Health Commission and Science and Technology Bureau) (No. 2020FYYX001, No. 2022MSXM149)."