Serum metabolomics study of narcolepsy type 1 based on ultra-performance liquid chromatography-tandem mass spectrometry

作者全名："Zhan, Qingqing; Wang, Lili; Liu, Nan; Yuan, Yuqing; Deng, Liying; Ding, Yongmin; Wang, Fen; Zhou, Jian; Xie, Liang"

作者地址："[Zhan, Qingqing; Yuan, Yuqing; Deng, Liying; Ding, Yongmin; Wang, Fen; Xie, Liang] Nanchang Univ, Dept Neurol, Affiliated Hosp 2, Nanchang 330006, Jiangxi, Peoples R China; [Wang, Lili; Liu, Nan; Zhou, Jian] Chongqing Med Univ, Inst Neurosci, Sch Basic Med Sci, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China"

通信作者："Xie, L (通讯作者)，Nanchang Univ, Dept Neurol, Affiliated Hosp 2, Nanchang 330006, Jiangxi, Peoples R China.; Zhou, J (通讯作者)，Chongqing Med Univ, Inst Neurosci, Sch Basic Med Sci, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China."

来源：AMINO ACIDS

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001120966700001

JCR分区：Q3

影响因子：3

年份：2023

卷号：55

期号：10

开始页：1247

结束页：1259

文献类型：Article

关键词：Narcolepsy type 1; Metabolite profile; Serum samples; Principal component analysis; Enrichment pathways

摘要："Narcolepsy is a chronic and underrecognized sleep disorder characterized by excessive daytime sleepiness and cataplexy. Furthermore, narcolepsy type 1 (NT1) has serious negative impacts on an individual's health, society, and the economy. Currently, many sleep centers lack the means to measure orexin levels in the cerebrospinal fluid. We aimed to analyze the characteristics of metabolite changes in patients with NT1, measured by ultra-performance liquid chromatography-tandem mass spectrometry. A principal component analysis (PCA), an orthogonal partial least square discriminant analysis (OPLS-DA), t tests, and volcano plots were used to construct a model of abnormal metabolic pathways in narcolepsy. We identified molecular changes in serum specimens from narcolepsy patients and compared them with control groups, including dehydroepiandrosterone, epinephrine, N-methyl-D-aspartic acid, and other metabolites, based on an OPLS-loading plot analysis. Nine metabolites yielded an area under the receiver operating curve > 0.75. Meanwhile, seven abnormal metabolic pathways were correlated with differential metabolites, such as metabolic pathways; neuroactive ligand-receptor interaction; and glycine, serine, and threonine metabolism. To our knowledge, this is the first study to reveal the characteristic metabolite changes in sera from NT1 patients for the selection of potential blood biomarkers and the elucidation of NT1 pathogenesis."

基金机构：National Natural Science Foundation of China

基金资助正文：We thank all participants who served as research participants and the National Natural Science Foundation of China for funding this study