Developmental endothelial locus-1 promotes osteogenic differentiation and alveolar bone regeneration in experimental periodontitis with type 2 diabetes mellitus

作者全名："Ma, Qian; Hu, Yiyao; Li, Han; Kuang, Yunchun; Li, Jie; Song, Jinlin"

作者地址："[Ma, Qian; Hu, Yiyao; Li, Han; Kuang, Yunchun; Li, Jie; Song, Jinlin] Chongqing Med Univ, Coll Stomatol, Chongqing, Peoples R China; [Ma, Qian; Hu, Yiyao; Li, Han; Kuang, Yunchun; Li, Jie; Song, Jinlin] Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Ma, Qian; Hu, Yiyao; Li, Han; Kuang, Yunchun; Li, Jie; Song, Jinlin] Chongqing Med Univ, Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China; [Li, Jie; Song, Jinlin] Chongqing Med Univ, Coll Stomatol, 426 Songshibei Rd, Chongqing 401147, Peoples R China"

通信作者："Li, J; Song, JL (通讯作者)，Chongqing Med Univ, Coll Stomatol, 426 Songshibei Rd, Chongqing 401147, Peoples R China."

来源：JOURNAL OF PERIODONTAL RESEARCH

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001125069100001

JCR分区：Q1

影响因子：3.4

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：developmental endothelial locus-1; human periodontal ligament stem cells; osteogenic differentiation; periodontitis; type 2 diabetes mellitus

摘要："ObjectivesThis study sought to explore the role of developmental endothelial locus-1 (DEL-1) in osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) and investigate the therapeutic effect of DEL-1 in ligature-induced experimental periodontitis with type 2 diabetes mellitus (T2DM).BackgroundT2DM is a significant risk factor for periodontitis. Treatment modalities for periodontitis with T2DM are being explored. DEL-1 is a versatile protein that can modulate the different stages of inflammatory diseases including periodontitis. The direct effect of DEL-1 on osteogenic differentiation of PDLSCs in periodontitis with T2DM is poorly understood.MethodsPrimary hPDLSCs were isolated from periodontal ligament tissue and identified by flow cytometry. In osteogenesis experiments, alkaline phosphatase (ALP), Alizarin Red staining and western blot were used to assess the osteogenic effect of DEL-1 on hPDLSCs in high glucose and inflammation environments. The mouse model of ligature-induced experimental periodontitis was established. H&E and Masson's trichrome staining were used to assess the change of periodontal tissue after local periodontal injection of DEL-1. Immunohistochemical staining was used to evaluate osteogenic-related protein expression.ResultshPDLSCs expressed mesenchymal stem cell (MSC)-specific surface markers and were negative for hematopoietic cell surface markers. hPDLSCs had the potential for multidirectional differentiation. DEL-1 could enhance the osteogenic differentiation of hPDLSCs in high glucose and inflammation environments, although it did not return to the control level. Histological staining showed that DEL-1 contributed to alveolar bone regeneration and osteogenic-related protein expression, but the degree of improvement in T2DM mice was lower than in non-T2DM mice.ConclusionsIn summary, we demonstrated that DEL-1 could promote osteogenic differentiation of hPDLSCs in high glucose and inflammation environment and rescue alveolar bone loss in experimental periodontitis with T2DM, which could provide a novel therapeutic target for periodontitis with T2DM."

基金机构："National Key Research and Development Program of China [U22A20314, 82071072]; National Natural Science Foundation of China [2022YFC2504200]; National Key R&D of Program of China [CSTB2022NSCQ-LZX0004]; Natural Science Foundation of Chongqing"

基金资助正文："This work was financially supported by funding from the National Natural Science Foundation of China (grant no. U22A20314, 82071072), the National Key R&D of Program of China (grant no. 2022YFC2504200), and the Natural Science Foundation of Chongqing (grant no. CSTB2022NSCQ-LZX0004)."