3D-printed PCL/β-TCP/CS composite artificial bone and histocompatibility study

作者全名："Zheng, Chao; Zhang, Mingman"

作者地址："[Zheng, Chao] Chongqing Med Univ, Dept Orthopaed, Childrens Hosp, Chongqing, Peoples R China; [Zhang, Mingman] Chongqing Med Univ, Dept Pediat Liver Transplantat, Childrens Hosp, 136 Zhongshan 2nd Rd, Chongqing 400014, Peoples R China; [Zheng, Chao; Zhang, Mingman] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Chongqing Engn Res Ctr Stem Cell Therapy, Minist Educ,Key Lab Child Dev & Disorders,Children, Chongqing, Peoples R China"

通信作者："Zhang, MM (通讯作者)，Chongqing Med Univ, Dept Pediat Liver Transplantat, Childrens Hosp, 136 Zhongshan 2nd Rd, Chongqing 400014, Peoples R China.; Zhang, MM (通讯作者)，Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Chongqing Engn Res Ctr Stem Cell Therapy, Minist Educ,Key Lab Child Dev & Disorders,Children, Chongqing, Peoples R China."

来源：JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001130343100003

JCR分区：Q1

影响因子：2.8

年份：2023

卷号：18

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Bone defect; Artificial bone; Calcium sulfate; Osteogenesis; 3D printing scaffolds

摘要："Background Tissue-engineered bone materials are an effective tool to repair bone defects. In this study, a novel biodegradable polycaprolactone (PCL)/beta-tricalcium phosphate (beta-TCP)/calcium sulfate (CS) composite scaffold was prepared by using three-dimensional (3D) printing technology.MethodsScanning electron microscopy, gas expansion displacement, and contact goniometry were used to examine the 3D-printed PCL/beta-TCP/CS composite scaffolds. The results showed that the PCL/beta-TCP/CS scaffolds possessed controllable porosity, hydrophobicity, biodegradability, and suitable apatite mineralization ability. To confirm the bone regenerative properties of the fabricated composite scaffolds, scaffold extracts were prepared and evaluated for their cytotoxicity to bone marrow mesenchymal stem cells (BMSCs) and their ability to induce and osteogenic differentiation in BMSCs.Results The PCL/beta-TCP/CS composite scaffolds induced a higher level of differentiation of BMSCs than the PCL scaffolds, which occurred through the expression of bone metastasis-related genes. The New Zealand white rabbit radial defect experiment further demonstrated that PCL/beta-TCP/CS scaffolds could promote bone regeneration.Conclusions In summary, the 3D-printed PCL/beta-TCP/CS composite porous artificial bone has good cytocompatibility, osteoinductivity, and histocompatibility, which make it an ideal bone material for tissue engineering."

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